Leaf tissue of G. turneri was shipped to DoveTail Genomics for DNA extraction and construction of Hi-C sequencing libraries. These Hi-C sequencing libraries were sequenced on the Illumina HiSeq 2500 (PE125 bp) at the BYU DNASC. Reads were mapped to the G. raimondii (Paterson et al. 2012) reference genome, and a scaffolded assembly was created for G. turneri by Dovetail Genomics. The initial assembly consisted of 12 scaffolds. Whole genome alignments identified in-silico assembly errors where a contiguous 25.7 MB of Chromosome 9 (D10_09) was initially placed on D10_12, and the reminder of that chromosome was in smaller scaffolded pieces. Similar to the process above, manual iterations of scaffolding correctly assembled D10_09 and D10_11 using Juicebox (Durand 2016). The final genome sequence of G. turneri was constructed using a custom python script developed by PhaseGenomics, LLC.