4.2. Analytical methods
Most analytical methods were described previously by Coronel et al.[37]. Cell density was estimated by OD at 750 nm and biomass dry weight, which was determined by centrifugation of culture samples at 3,900 x g for 10 min and drying out the cell pellets in an oven at 70 °C until constant weight (2 – 3 days). For the determination of total biomass carbohydrates, samples were reacted with the anthrone reagent [40] and carbohydrates content was calculated from a standard curve made of glucose. Lipids were determined by the sulfo-phospho-vanillin method[41] using canola oil as a lipid standard.