Cell culture
Ovarian cancer cell line (SKOV3) was purchased from ECACC (Wiltshire, UK). SKOV3 cells were cultured in Gibco RPMI media 1640 (ThermoFisher, Paisley, UK) supplemented with 10% foetal bovine serum (FBS) and 1% penicillin-streptomycin (ThermoFisher, Paisley, UK) at 37°C with 5% CO2 and balanced with air. SKOV3 cells were administered with 2.5% sevoflurane (ABOTT, Sittingbourne, UK), 4 μg/mL propofol (Sigma-Aldrich, Dorset, UK), media (naïve control), or intralipid (vehicle control) (Santa Cruz Biotechnology, Dallas, Texas, USA) for 2 hours, and followed by 24 hours recovery in fresh media.