Fig 2. The effects of sevoflurane and propofol on the expressions of GLUT1, MPC1, and GLUD1 in ovarian cancer cells.
SKOV3 cells were exposed to media (naïve control), 2.5% sevoflurane, or 4 μg/mL propofol. The expressions of GLUT1 (A) and MPC1 (B) were detected with immunofluorescent staining. The expression levels of these two markers were validated with Western blot analysis and the expression level of GLUD1 was also analysed (C). The intensity of the bands in Western blot analysis was normalised by housekeeping protein GAPDH. The data was analysed with one-way ANOVA and Dunnett multi-comparison test (D-F) and was presented as mean ± standard deviation and dots plot (n = 6). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 versus naïve control. Scale bar: 50 μm. NC: naïve control group; S: sevoflurane group; P: propofol group; GLUT1: glucose transporter 1; MPC1: mitochondrial pyruvate carrier 1; GLUD1: glutamate dehydrogenase 1; ANOVA: analysis of variance.