Effects of sevoflurane or propofol on the cellular
metabolism of SKOV3 cells
The metabolic profiles obtained from media samples of naïve control,
vehicle control, sevoflurane, and propofol groups were analysed using
unsupervised PCA analysis with 2 principal components. The PCA scores
plot showed a clear grouping pattern among these four groups
(Fig 3A ). Pair-wise comparisons of naïve control vs .
sevoflurane group and vehicle control vs . propofol group were
carried out using OPLS-DA analysis with one predictive component and one
orthogonal component. Permutation p values showed both models of naïve
control vs . sevoflurane (p = 0.005) and vehicle controlvs . propofol (p = 0.005) had significances. The permutation p
values together with R2X, Q2X, and
Q2Y of two models were summarised (Table 1 ).
The OPLS-DA scores plots showed there were clear separations between
naïve control and sevoflurane group (Fig 3B ), and vehicle
control and propofol group (Fig 3D ). The OPLS-DA loadings plots
showed that the concentrations of glucose and glutamine were decreased,
and the concentration of isopropanol was increased in the sevoflurane
group compared to the naïve control group (Fig 3C ). However,
the concentrations of glucose and glutamine were increased, and the
concentration of isopropanol was decreased in the propofol group in
contrast to vehicle control group (Fig 3E ). After anaesthetics
administration, the concentrations of lactate, pyruvate, acetate,
alanine, valine, and leucine were increased by both sevoflurane and
propofol. Furthermore, propofol also increased the levels of glycerol,
fatty acids, asparagine, succinate, acetone, arginine, and isoleucine,
but decreased the level of ethanol (Table 2 ).