Effects of sevoflurane or propofol on the expressions of GLUT1, MPC1, and GLUD1 in ovarian cancer cells
Based on the immunofluorescent staining of cells, the intensities of GLUT1 (Fig 2A ) and MPC1 (Fig 2B ) were higher in the sevoflurane group compared to naïve control, but much lower in the propofol group than the naïve control. From Western blot analysis, the protein expression levels of GLUT1 (NC vs . S, 1.0 ± 0.1vs . 1.4 ± 0.3, p < 0.01, n = 6) and MPC1 (NC vs . S, 1.0 ± 0.3 vs . 1.5 ± 0.2, p < 0.01, n = 6) were significantly increased after sevoflurane exposure (Fig 2C ). However, the expression levels of GLUT1 (NC vs . P, 1.0 ± 0.1vs . 0.3 ± 0.1, p < 0.0001, n = 6) and MPC1 (NCvs . P, 1.0 ± 0.3 vs . 0.6 ± 0.3, p < 0.05, n = 6) were significantly decreased after propofol treatment compared to the naïve control (Fig 2E and F ). Similarly, the expression level of glutamate dehydrogenase 1 (GLUD1) was significantly increased by sevoflurane administration (NC vs . S, 1.0 ± 0.1 vs . 1.4 ± 0.1, p < 0.01, n = 6), but decreased by propofol administration (NC vs . P, 1.0 ± 0.1 vs . 0.6 ± 0.2, p < 0.001, n = 6) (Fig 2D ).