Effects of sevoflurane or propofol on the expressions of
GLUT1, MPC1, and GLUD1 in ovarian cancer cells
Based on the immunofluorescent staining of cells, the intensities of
GLUT1 (Fig 2A ) and MPC1 (Fig 2B ) were higher in the
sevoflurane group compared to naïve control, but much lower in the
propofol group than the naïve control. From Western blot analysis, the
protein expression levels of GLUT1 (NC vs . S, 1.0 ± 0.1vs . 1.4 ± 0.3, p < 0.01, n = 6) and MPC1 (NC vs .
S, 1.0 ± 0.3 vs . 1.5 ± 0.2, p < 0.01, n = 6) were
significantly increased after sevoflurane exposure (Fig 2C ).
However, the expression levels of GLUT1 (NC vs . P, 1.0 ± 0.1vs . 0.3 ± 0.1, p < 0.0001, n = 6) and MPC1 (NCvs . P, 1.0 ± 0.3 vs . 0.6 ± 0.3, p < 0.05, n = 6)
were significantly decreased after propofol treatment compared to the
naïve control (Fig 2E and F ). Similarly, the expression level
of glutamate dehydrogenase 1 (GLUD1) was significantly increased by
sevoflurane administration (NC vs . S, 1.0 ± 0.1 vs . 1.4 ±
0.1, p < 0.01, n = 6), but decreased by propofol
administration (NC vs . P, 1.0 ± 0.1 vs . 0.6 ± 0.2, p
< 0.001, n = 6) (Fig 2D ).