Cell migration was assessed with wound healing assay
SKOV3 cells were seeded in petri dishes to form a continuous monolayer.
Cancer cells were incubated in pure media, 2.5% sevoflurane, or 4 μg/mL
propofol for 2 hours. The monolayer of cancer cells was scratched to
form a cell-free gap. The petri dishes were washed with PBS to discard
suspended cancer cells. The cell-free gap was captured under a
microscope as the baseline. After incubated with culture media for 24
hours, the cell-free gap was captured again under the microscope to
calculate the migrate capability of cancer cells with Fiji (ImageJ 2.0)
software (National Institutes of Health, Bethesda, Maryland, USA).