Materials
The SKOV3 cell line was purchased from ECACC, Wiltshire, UK. The Gibco
RPMI media 1640, penicillin-streptomycin, methanol, NuPAGE LDS sample
buffer, NuPAGE Bis-Tris gel and nitrocellulose membrane were obrained
from ThermoFisher, Paisley, UK. The sevoflurane was purchased from
ABOTT, Sittingbourne, UK. The propofol, Triton X-100, donkey serum and
crystal violet were acquired from Sigma-Aldrich, Dorset, UK. The
intralipid, PBS, paraformaldehyde, anti-Ki-67 antibody and luminol
reagent solution were from Santa Cruz Biotechnology, Dallas, Texas, USA.
The anti-rabbit and anti-mouse antibodies for immunofluorescent
staining, anti-GLUT1, anti-GLUD1, anti-PEDF, anti-CXCL12 and anti-CXCR4
antibodies were obtained from Abcam, Cambridge, UK. The DAPI mounting
media was acquired from Vector Laboratories, Burlingame, California,
USA. The Corning Matrigel matrix was purchased from Corning, New York,
USA. The anti-MPC1 and anti-HIF-1α antibodies were obtained from Novus
Biologicals, Abingdon, UK. The anti-p-Erk1/2 and anti-Erk1/2 antibodies,
anti-rabbit and anti-mouse antibodies for Western blot analysis, and
cell lysis buffer were acquired from Cell signalling Technology, London,
UK. The anti-GAPDH antibody was purchased from Merck, Hertfordshire, UK.
The NMR tube was from Bruker Corporation, Rheinstetten, Germany.