Fig 3. The metabolomics study of sevoflurane- or
propofol-treated ovarian cancer cells using 1H-NMR
spectroscopy.
Ovarian cancer cells were administered with media (naïve control),
intralipid (vehicle control), 2.5% sevoflurane or 4 μg/mL propofol for
2 hours plus 24 hours recovery time. After treatment, the culture media
of ovarian cancer SKOV3 cells was collected for 1H-NMR
spectroscopy experiment. PCA scores plot of 1H-NMR
spectra of media was shown (A). R2X represented the
fraction of variance in the 1H-NMR data modelled by
two principal components (t[1] and t[2]). The OPLS-DA scores
plots (B: NC vs . S; D: VC vs . P) and loadings plots (C: NCvs . S; E: VC vs . P) were derived from1H-NMR spectral data (n = 10). The colour bar
indicated the correlation coefficient values (r2) to
be high in red and low in blue. NC: naïve control group; VC: vehicle
control group; S: sevoflurane group; P: propofol group; PCA: principal
component analysis; OPLS-DA: orthogonal projection to latent
structures-discriminant analysis; Glc: glucose; Lac: lactate; Pyr:
pyruvate; Gln: glutamine; Ace: acetate; Ala: alanine; IPA: isopropanol;
Val: valine; Leu: leucine; Eth: ethanol; Gro: glycerol; Asn: asparagine;
FA: fatty acids; Suc: succinate; Acn: acetone; Arg: arginine; Ile:
isoleucine.