5) Optimizing sonication with master plots for common cell extract
parameters
Noting that both software access and computation time necessary to run
our finite element model may limit its utility, we also devised a set of
master plots that can likewise be used to efficiently select sonication
conditions for cell extract processing, but still gives the flexibility
to use strains that have different temperature and power density
limitations. Here we model the maximum temperature that can be reached
in aqueous samples by a standard tip sonication set up (cooling in
ice-bath with sufficiently large volume) for four common sonication tube
sizes, while varying power density and pulse settings (Fig 6). These
plots can be used directly to find optimal sonication parameters at a
set temperature threshold without having to run the FE model.