Statistical analysis
Mann-Whitney test was used to compare TMB between groups. The Chi-square test or Fisher’s exact test was used to test frequency between groups. All statistical analysis were carried out using GraphPad Prism software (v. 8.0.2; GraphPad Software, La Jolla, CA, USA) and R 4.0.2 software (R Foundation for Statistical Computing, Vienna, Austria). Statistical significance was defined as a two-sided p-value of <0.05).