2. Materials and Methods
The following chemicals were obtained from commercial sources and used
as received. Soluble type I collagen from calf skin was purchased from
Symatese (Chaponost, France). Agarose, β-Agarase from Pseudomonas
atlantica, FITC-labelled Hyaluronic acid, Transferrin-TRITC, Dulbecco’s
Modified Eagle Medium high glucose (DMEM), fetal bovine serum (FBS),
penicillin and streptomycin were purchased from Sigma–Aldrich, Italy.
Live-Dead assay and MitoTracker Red CMXRos were purchased from Thermo
Fisher. Ultrapure grade water with a conductivity of 18.2 MΩ cm was used
in all experiments.