2.4 Diffusion test
PBS solutions containing either FITC-labelled Hyaluronic acid (10kDa
molecular weight) or Transferrin-TRITC (80 kDa molecular weight) at
known concentration were prepared and deposited over the A-C hydrogels.
The photoluminescence signal of the loaded solutions was recorded over
time and the resulting concentration was extrapolated from a calibration
curve. The diffusivity of the two molecules within the hydrogels was
assessed determining the ratio between the concentration of the
fluorescent molecule (either hyaluronic acid or transferrin) at the
analyzed time point and that measured in free PBS.