2.8 Enzymatic digestion of agarose for spheroids recovery
0.25%-0.02% and 0.125%-0.02% A-C hydrogels containing MCF-7
spheroids at different days of growth were incubated with 40 U of
β-Agarase from Pseudomonas atlantica and left under incubation O.N. at
37 °C. Then, the supernatant was collected and observed under an optical
microscope to visualize the presence of floating spheroids. Soonafter,
the spheroids were fixed with 4% paraformaldehyde and stained with
DAPI. The labelled structures were imaged under a Fluorescence
Microscope (EVOS FLoid Cell Imaging Station, ThermoFisher, Waltham, MA
USA).