2.8 Enzymatic digestion of agarose for spheroids recovery
0.25%-0.02% and 0.125%-0.02% A-C hydrogels containing MCF-7 spheroids at different days of growth were incubated with 40 U of β-Agarase from Pseudomonas atlantica and left under incubation O.N. at 37 °C. Then, the supernatant was collected and observed under an optical microscope to visualize the presence of floating spheroids. Soonafter, the spheroids were fixed with 4% paraformaldehyde and stained with DAPI. The labelled structures were imaged under a Fluorescence Microscope (EVOS FLoid Cell Imaging Station, ThermoFisher, Waltham, MA USA).