2.4 Diffusion test
PBS solutions containing either FITC-labelled Hyaluronic acid (10kDa molecular weight) or Transferrin-TRITC (80 kDa molecular weight) at known concentration were prepared and deposited over the A-C hydrogels. The photoluminescence signal of the loaded solutions was recorded over time and the resulting concentration was extrapolated from a calibration curve. The diffusivity of the two molecules within the hydrogels was assessed determining the ratio between the concentration of the fluorescent molecule (either hyaluronic acid or transferrin) at the analyzed time point and that measured in free PBS.