2. Materials and Methods
The following chemicals were obtained from commercial sources and used as received. Soluble type I collagen from calf skin was purchased from Symatese (Chaponost, France). Agarose, β-Agarase from Pseudomonas atlantica, FITC-labelled Hyaluronic acid, Transferrin-TRITC, Dulbecco’s Modified Eagle Medium high glucose (DMEM), fetal bovine serum (FBS), penicillin and streptomycin were purchased from Sigma–Aldrich, Italy. Live-Dead assay and MitoTracker Red CMXRos were purchased from Thermo Fisher. Ultrapure grade water with a conductivity of 18.2 MΩ cm was used in all experiments.