Pathological and immunohistochemistry examination of ovarian teratoma tissue
The tissue of teratoma was fixed by 4% paraformaldehyde, embedded by paraffin and sliced using a microtome. The sections were deparaffinized, dehydrated and subjected to hematoxylin and eosin staining.
Immunohistochemical analyses of teratoma tissue were performed using a rabbit polyclonal antibody against LGI1 (diluted 1:200, BIOSS, Beijing, China). The immunostaining protocol for LGI1 detection were described previously[16].
The study was approved by the HUST Committee on Human Research and all patients signed informed content to participate.
Results