Pathological and immunohistochemistry examination of ovarian
teratoma tissue
The tissue of teratoma was fixed by 4% paraformaldehyde, embedded by
paraffin and sliced using a microtome. The sections were deparaffinized,
dehydrated and subjected to hematoxylin and eosin staining.
Immunohistochemical analyses of teratoma tissue were performed using a
rabbit polyclonal antibody against LGI1 (diluted 1:200, BIOSS, Beijing,
China). The immunostaining protocol for LGI1 detection were described
previously[16].
The study was approved by the HUST Committee on Human Research and all
patients signed informed content to participate.
Results