Animals
CCR2 knockout (KO) mice on C57BL/6 (B6) background were purchased from
Jackson laboratory (Bar Harbor, ME, USA). Wildtype (WT) C57BL/6J
(CD45.2+ and CD45.1+) mice were
purchased from Charles River (Beijing, China). CCR2 KO mice displayed a
stable phenotype based on sufficient backcrossing with B6 mice. Mice
were bred and placed in a specific-pathogen–free animal department.
Male and female mice between 2-6 months old were used for the
experiments. All studies were conducted in a non-blinded fashion. Animal
experimentation was reviewed and approved by the Institutional Animal
Care and Ethics Committee of Animal Experimentation of Tongji Medical
College.