In vitro inhibitory treatment with Rapamycin, XMU-MP-1,
and Fludarabine
Purified CCR2 KO B cells were pretreated with 20 nM rapamycin (HY-10219,
MedChem Express, Monmouth Junction, NJ, USA), 3 μM XMU-MP-1 (T4212,
TargetMol, Boston, MA, USA) or 5 μg fludaradine (T1038, TargetMol) and
incubated at 37 °C for 2 h before being activated with sAg. The
remaining steps were the same as the untreated control.