A soil sampler was used to collect the soil samples (5 × 5 × 20 cm; length × width × depth). The samples were transported to the laboratory (<2 h) and stored at - 80 °C until DNA extraction. An organic (0-10 cm) and a mineral (10-20 cm) lay separations were performed in the laboratory for each biological replica (the core of the soil). Sampled soil was sifted (2 mm mesh) and homogenized prior to molecular analysis for each replica, while replicas were grouped for soil analysis (Uroz et al. 2013).