Statistical analyses
All statistical analyses were performed in R (v.3.4.3; R Core Team
2018). Phyloseq package (McMurdie et al. 2013). “Quantitative Insights
into Microbial Ecology 2” (QIIME 2 v2018.6) (Caporaso et al. 2010).
VSEARCH (Rognes et al. 2016). The rarefied data was used to calculate if
the altitude gradient was significantly correlated with the fungi
composition. For this purpose, Bray-Curtis distance calculation was
applied, and permutation was set to 999. PERMANOVA analysis was
calculated using the vegan R package (Oksanen et al. 2015).