Figure2. Spotting test
5 out of 12 yeast samples in complex backgrounds or not were taken and diluted to 2×107 cells per ml, and then the diluent samples were subjected to 10-fold dilutions. 5 μl of each dilution were spotted onto YPD solid media. Thus, the numbers at the top of images indicate the CFUs in theory. (A) Manual counting results for samples of simple backgrounds. (B) Automatic counting results for samples of simple backgrounds with “Yeast Counter”. (C) Manual counting results for samples of complex backgrounds. (D) Automatic counting results with ilastik and ImageJ for samples under complex backgrounds.