Comparison of NETs and C5a between the patient and HC group
The NETs and C5a levels in peripheral blood from all patients and
controls were compared by the two-tailed unpaired t test with
Welch’s correction. It was found to that both the variables in patient
group were
significantly
higher than that in HC group (0.62 ± 0.22 vs. 0.26 ± 0.15,p <0.001 and 4.64 ± 1.04ng/ml vs. 2.88 ±
0.96ng/ml, p <0.001, respectively) (Fig. 2A and Fig.
2B).
Analysis of the relationships in laboratoryvariables
in patient group
It was showed by Spearman correlation analysis that PAD4 expressions in
neutrophil, which included the percentage of neutrophils expressed PAD4
in all neutrophils and MFI of PAD4+neutrophils, were
correlated with NETs (r =0.432, p =0.0054 andr =0.395, p =0.0117, respectively) (Fig.3A and Fig. 3B). In
addition, NETs displayed a statistically significant correlation with
MPO-ANCA but not with C5a (r =0.415, p =0.0078 andr =0.270, p =0.0920, respectively) (Fig. 3C, Fig. 3D).
Furthermore, MPO-ANCA exhibited significant correlation with C5a
(r =0.558, p =0.0002) (Fig. 3E).