5. Conclusion
The effect of slope gradient and slope position on soil bacterial and fungal diversities and community compositions in black soil were analyzed using the 16S and ITS rRNA Illumina sequencing. The dominant phyla were Proteobacteria, Acidobacteria, Gemmatimonadetes, Planctomycetes, and Actinobacteria, representing the most dominant phyla of bacteria. The most abundant phyla for the fungal community were Ascomycota, Anthophyta, Basidiomycota, Chlorophyte, and Chytridimycota. Microbial alpha-diversity (Bacteria and Fungi) was similar among the slope gradients or slope positions. The bacterial and fungal community composition was only affected by slope gradient, which was significantly lower in the highest slope gradient level than others. This study suggests that slope gradient but not slope position affecting microbial community composition in the sloped cropland area.
Acknowledgement We wish to thank the National Key Research and Development Project (Grant No. 2018YFC0507005), National Natural Science Foundation of China (Grant No. 32071840) and National Natural Science Foundation of China (Grant No. 31070627) for funding this research. Abdul Hakim Jamshidi and Lei Sun contributed equally to this work. We further thanks to Yannan Xu, Xiang Li, Xiangwei Yang, Wentao Di, Wenjuan Huo and many others for assisting with soil sample collection and analyses. We are extremely appreciative of the help given by Gene Denovo Corp. during microbial community analysis.
Table 1 Effect of slope gradient and slope position on physico-chemical and biological properties.