5. Conclusion
The effect of slope gradient and slope position on soil bacterial and
fungal diversities and community compositions in black soil were
analyzed using the 16S and ITS rRNA Illumina sequencing. The dominant
phyla were Proteobacteria, Acidobacteria, Gemmatimonadetes,
Planctomycetes, and Actinobacteria, representing the most dominant phyla
of bacteria. The most abundant phyla for the fungal community were
Ascomycota, Anthophyta, Basidiomycota, Chlorophyte, and Chytridimycota.
Microbial alpha-diversity (Bacteria and Fungi) was similar among the
slope gradients or slope positions. The bacterial and fungal community
composition was only affected by slope gradient, which was significantly
lower in the highest slope gradient level than others. This study
suggests that slope gradient but not slope position affecting microbial
community composition in the sloped cropland area.
Acknowledgement We wish to thank the National Key Research and Development Project
(Grant No. 2018YFC0507005), National Natural Science Foundation of China
(Grant No. 32071840) and National Natural Science Foundation of China
(Grant No. 31070627) for funding this research. Abdul Hakim Jamshidi and
Lei Sun contributed equally to this work. We further thanks to Yannan
Xu, Xiang Li, Xiangwei Yang, Wentao Di, Wenjuan Huo and many others for
assisting with soil sample collection and analyses. We are extremely
appreciative of the help given by Gene Denovo Corp. during microbial
community analysis.
Table 1 Effect of slope gradient and slope position on physico-chemical
and biological properties.