Data and Statistical analysis
All analysis were performed using Prism 6.0 software (GraphPad, USA) and
followed the guidelines on experimental design and analysis in
pharmacology (Curtis et al., 2018). Data are expressed as the mean ±
standard error of the mean (SEM). The paired Student’s t test was
used to compare basal values of MAP, HR, tail skin temperature and
spontaneous baroreflex analysis before and after drug treatment. We used
the unpaired Student’s t test to compare baseline corticosterone
values (before stress) between the control and drug treatments. Changes
in cardiovascular parameters, tail skin temperature, spontaneous
baroreflex and corticosterone levels evoked by restraint stress were
analysed using two-way ANOVA with treatment as the main factor (vehicle
x drugs) and time as repeated measures followed by Bonferroni’spost hoc test. P<0.05 was set as significant.