Drugs and solutions
NBQX (Tocris Bioscience, USA; selective non-NMDA glutamate receptor
antagonist) and LY235959 (Tocris Bioscience, USA; selective NMDA
glutamate receptor antagonist) were dissolved in artificial
cerebrospinal fluid (aCSF) (100 mM NaCl; 2 mM
Na3PO4; 2.5 mM KCl; 1.0 mM
MgCl2; 27 mM NaHCO3; 2.5 mM
CaC2; pH 7.4). Urethane (Sigma, USA) and
2,2,2-tribromoethanol (Sigma, USA) were dissolved in saline (0.9%
NaCl). The veterinary pentabiotic (Fort Dodge, Campinas, SP, Brazil) and
the non-steroidal anti-inflammatory drug flunixin meglumine (Banamine®,
Schering-Plough, Brazil) were used as provided.