Conclusion
Among 795 Nsp1 substitutions, 69 belonged to the HTH motif in the C-terminal region. In this article, out of 69 mutations, only 11 substitutions with a frequency above 10 were examined and it was revealed that these mutations affect the thermodynamic parameters of the interaction between the protein and the ribosome. The effect of HTH mutations may increase the pathogenicity of SARS-CoV-2 and in turn, increase the inhibition of host protein translation. Therefore, findings suggest that Nsp1 should be considered as an important virulence factor because more mutations in the binding motif of Nsp1 may have drastic consequences.