Conclusion
Among 795 Nsp1 substitutions, 69 belonged to the HTH motif in the
C-terminal region. In this article, out of 69 mutations, only 11
substitutions with a frequency above 10 were examined and it was
revealed that these mutations affect the thermodynamic parameters of the
interaction between the protein and the ribosome. The effect of HTH
mutations may increase the pathogenicity of SARS-CoV-2 and in turn,
increase the inhibition of host protein translation. Therefore, findings
suggest that Nsp1 should be considered as an important virulence factor
because more mutations in the binding motif of Nsp1 may have drastic
consequences.