2.4. Nsp1 intrinsically disordered regions (IDRs)
PONDR was used to investigate intrinsically disordered regions (IDRs) of SARS-CoV-2 Nsp1, which is based on several different predictors such as VL3 and VSL2 [34,35]. In each of these predictors, the first letter refers to the method of characterization (V: Various) and the second letter refers to the length of the disordered region [S: Short (8 - 9 residues), and L: Long (40 or more residues)].