2.11 Sucrose Preference Test (SPT)
This test lasted for 4 days and
was carried out according to previously reported methods with some
modifications(Li-Wen et al., 2020). On the first 2 days, the C57BL/6J
mice were free to drink two bottles containing pure water and 1%
sucrose solution, respectively. And the position of two bottles was
changed every 6 h to prevent potential location preference of drinking.
On the 3rd day, both the food and two bottles were deprived for 24
hours. On the 4th days, the test lasted for 6 hours, with the position
of the bottles interchanged and the two bottles weighed before and after
the test period. Sucrose preference was calculated as the percentage of
sucrose solution ingested relative to the total amount of liquid intake.
Drug treatments were not given during the testing days.