3Department of Community Medicine and Public
Health, Faculty of Medicine, Alexandria University, Egypt.
Affiliation: Alexandria University Faculty of Medicine, Egypt
Background : Gut microbiota cluster into three enterotypes named
the Bacteroides , Prevotella and Ruminococcus . While
each person’s microbial “fingerprint” is unique, there are specific
patterns seen in those that are healthy and those that have specific
illnesses. The aim of the present study is to identify the enterotypes
that are likely related to Multiple Sclerosis Egyptian patients as well
as their possible role in the course of the disease. Subjects &
Methods: Thirty patients with remitting relapsing multiple sclerosis,
who presented to the MS Clinic of Alexandria University Hospital were
enrolled in our study. These were diagnosed according to according to
McDonnald 2017 criteria. A cross matching control group of 20 healthy
subjects of similar age and sex were included. Stool specimens were
taken from each. Quantitative SYBR Green Real-Time PCR was done for the
identification and quantitation of Bacteroides, Prevotella andRuminococcus which constitute the core of the three major
enterotypes. Results: Enterotype 1 is the most common
enterotype detected in MS and control cases (80% versus 65%). For
Enterotype 3, it was not detected in any of the 20 control cases while
detected in multiple sclerosis case (16.7%). However, by comparing the
multiple sclerosis and control cases Enterotype 2 is significantly less
in multiple sclerosis than control (3.3% versus 35%).Conclusion: Although Enterotype 2 is significantly less in
multiple sclerosis patients, collapsing the whole microbiome variations
into dominant enterotypes was not appropriate to identify disease
association or to be used as a disease biomarker.
Keywords : gut microbiome; multiple sclerosis; Real Time PCR;
16S rRNA; dysbiosis; enterotypes
Corresponding author
Email:
Shwikar.elsalam@alexmed.edu.eg
ORCID ID: https://orcid.org/0000-0002-9390-0117
Mobile number: +2 01273786946