Figure 4. CsHSFAs regulate CsJAZ6 expression.
(a) Yeast one-hybrid assay to confirm binding of CsHSFAs to theCsJAZ6 promoter. (b-c) EMSA assay to validate the binding of CsHSFA1b and CsHSFA2 to HSE motifs (TCCNNGAA) in theCsJAZ6 promoter. (d) Structural schematics of the effector (35S:CsHSFA1b and 35S:CsHSFA2 ) and reporter (CsJAZ6Pro::LUC ) constructs used in the dual-luciferase transient expression assay. (e) CsHSFA1b and CsHSFA2 activate the transcription activity of the CsJAZ6 promoter. Relative firefly luciferase (LUC) to Renilla luciferase (REN) ratios in tobacco leaves co-transfected with the CsJAZ6 reporter and CsHSFA1b/CsHSFA2 effector constructs. Error bars indicate the SD of three biological replicates.