Bimolecular fluorescence complementation (BiFC) assay
The full-length cDNA sequences of CsJAZ6 , CsEGL3 , andCsTTG1 were cloned into the cYFP and nYFP vectors. Six fusion constructs obtained, including CsJAZ6-YFPN, CsJAZ6-YFPC, CsEGL3-YFPN, CsEGL3-YFPC, CsTTG1-YFPN, and CsTTG1-YFPC, were transformed into Agrobacterium tumefaciens strain GV3101. CsJAZ6-YFPN was paired with CsEGL3/CsTTG1-YFPC and CsJAZ6-YFPC with CsEGL3/CsTTG1-YFPNfor transient coexpression in tobacco leaves. The YFP fluorescence signal for each combination was imaged at 44-48 h after infiltration using a Leica TCS SP5 confocal laser scanning microscope system (Leica Microsystems, Bannockburn, IL, USA). An excitation wavelength of 488 nm and fluorescence detection at 500-542 nm were used.