Figure 3. Effect of JA treatment on catechins in tea leaves under HT.
(a) , The effect of HT on expression of genes in the JA synthesis and signaling pathways. Error bars indicate the SD of three biological replicates. (b) MeJA accumulation in tea leaves under HT. Error bars indicate the SD of three biological replicates.(c) Catechin content in tea leaves treated with MeJA, Ibu, or MeJA+MG132 and then transfer to grown under normal (25 ℃) or high (40 ℃) temperature. Different letters above the bars indicate significant differences between groups (P<0.05; ANOVA with Fisher’s LSD test). (d) qPCR determination of the transcript levels ofCsCHS , CsDFR , and CsANS in tea leaves treated with MeJA, Ibu, or MeJA+MG132 and then transfer to grown under normal (25 ℃) or high (40 ℃) temperature. Different letters above the bars indicate significant differences between groups (P<0.05; ANOVA with Fisher’s LSD test).