Figure 8. CsHSFAs mediate the effect of HT on anthocyanin biosynthesis in Arabidopsis.
(a-b) Phenotype and anthocyanin content of detached leaves of Wild-type (Col-0), Pro35S:CsHSFA1b (CsHSFA1bOE2/OE3 ), andPro35S:CsHSFA2 (CsHSFA2OE2/OE3 ) plants that grown under normal (22 ℃) or high (40 ℃) temperature. Different letters above the bars indicate significant differences between groups (P<0.05; ANOVA with Fisher’s LSD test). (c) Gene expression ofAtCHS , AtDFR and AtANS in Wild-type (Col-0),Pro35S:CsHSFA1b (CsHSFA1bOE2/OE3 ), andPro35S:CsHSFA2 (CsHSFA2OE2/OE3 ) plants that grown under normal (22 ℃) or high (40 ℃) temperature. Different letters above the bars indicate significant differences between groups (P<0.05; ANOVA with Fisher’s LSD test). (d) MeJA content of detached leaves in Wild-type (Col-0), Pro35S:CsHSFA1b(CsHSFA1bOE2/OE3 ), and Pro35S:CsHSFA2(CsHSFA2OE2/OE3 ) plants that grown under normal (22 ℃) or high (40 ℃) temperature. Different letters above the bars indicate significant differences between groups (P<0.05; ANOVA with Fisher’s LSD test).