Supplemental Data
Supplemental Figure S1. Yeast one-hybrid assay to confirm
binding of CsHSFAs to the CsJAZ6 promoter.
Supplemental Figure S2. Gene expression of AtCHS ,AtDFR , and AtANS in leaves of Arabidopsis. (a)qPCR analysis of the transcript levels of AtCHS , AtDFR ,
and AtANS in leaves of Col-0 and athsfa2 plants. Error
bars indicate the SD of three biological replicates. (b) qPCR
analysis of the transcript levels of AtCHS , AtDFR , andAtANS in leaves of Col-0 that grown under normal (22 ℃) or high
(40 ℃) temperature. Error bars indicate the SD of three biological
replicates.
Supplemental Figure S3. Gene expression of CsHSFA1b andCsHSFA2 in over-expressed lines Col-0, athsfa2 and
over-expressed plants. (a) qPCR analysis of the transcript
levels of CsHSFA1b in Col-0, athsfa2, andPro35S:CsHSFA1b/ Col-0 (CsHSFA1b/ Col-0 T2 1-3) andPro35S:CsHSFA1b/athsfa2 (CsHSFA1b/athsfa2 T2 1-3) transgenic
plants. Error bars indicate the SD of three biological replicates.(b) qPCR analysis of the transcript levels of CsHSFA2 in
Col-0, athsfa2, and Pro35S:CsHSFA2/ Col-0(CsHSFA2/ Col-0 T2 1-3) and Pro35S:CsHSFA2/athsfa2
(CsHSFA2/athsfa2 T2 1-3) transgenic plants. Error bars indicate the SD
of three biological replicates.
Supplemental Figure S4. CsHSFAs mediate the effect of HT on
anthocyanin biosynthesis in Arabidopsis .
(a-b) Phenotype and anthocyanin content of detached leaves ofathsfa2 , Pro35S:CsHSFA1b/athsfa2 (CsHSFA1b/athsfa2
T2 2-3 ) and Pro35S:CsHSFA2/athsfa2 (CsHSFA2/athsfa2 T2
2-3 ) plants that grown under normal (22 ℃) or high (40 ℃) temperature.
Different letters above the bars indicate significant differences
between groups (P<0.05; ANOVA with Fisher’s LSD test).(c) Gene expression of AtCHS , AtDFR , andAtANS in athsfa2 , Pro35S:CsHSFA1b/athsfa2(CsHSFA1b/athsfa2 T2 2-3 ), and Pro35S:CsHSFA2/athsfa2(CsHSFA2/athsfa2 T2 2-3 ) plants that grown under normal (22 ℃) or
high (40 ℃) temperature. Different letters above the bars indicate
significant differences between groups (P<0.05; ANOVA with
Fisher’s LSD test). (d) MeJA content in detached leaves ofathsfa2 , Pro35S:CsHSFA1b/athsfa2 (CsHSFA1b/athsfa2
T2 2-3 ), and Pro35S:CsHSFA2/athsfa2(CsHSFA2/athsfa2 T2 2-3 ) plants that grown under normal (22 ℃) or
high (40 ℃) temperature. Different letters above the bars indicate
significant differences between groups (P<0.05; ANOVA with
Fisher’s LSD test).
Table S1 Oligonucleotide primer sequences