Anthocyanin and Catechin determination
Arabidopsis leaves were weighed (0.1 g) and incubated overnight
in extraction buffer (methanol containing 1% HCl). Anthocyanins were
separated from the chloroform by adding 200 µl distilled water and 500
µl chloroform, as previously described by He et al47.
Spectrophotometer was used to measure the total anthocyanins in the
aqueous phase with the absorbance at 530 and 657 nm using. The number of
anthocyanins per gram fresh weight was calculated as
(A530-0.25×A657). A modified protocol
was further used to detect the Catechin content (Su et al., 2020).