Transient gene suppression in tea plants
Gene suppression assays were according to previous study from Hu et al
(Hu et al., 2022). CsHSFA1b , CsHSFA2 , and CsJAZ6were used as input sequences to design candidate antisense
oligonucleotides (AsODNs) with the Soligo software (Ding and Lawrence,
2003) (Table S1). At least ten individual plants were infiltrated with
gene-specific AsODNs; as controls, untreated plants grown under the same
conditions and infiltrated with corresponding sense oligonucleotides
(sODNs). The leaves were harvested after 24 h treatment.