7. MECP2 and IRAK1 genes spanning the Xq28 duplication were not upregulated by RNA-seq and RT-PCR
There were transcriptions of genes spanning the Xq28-duplicated region in the peripheral mononuclear blood cells (PMBCs) by RNA-seq. Expression dosages of the MECP2 and IRAK1 genes showed no significant difference between II2 and I2 (Supp. Table S6). We also detected the mRNA of MECP2 and IRAK1 in the two females and three normal females by RT-PCR (Figure 5). The expression dosages ofMECP2 and IRAK1 in the Xq28-duplication females were not upregulated compared to those of the normal females.