7. MECP2 and IRAK1 genes spanning the Xq28
duplication were not upregulated by RNA-seq and RT-PCR
There were transcriptions of genes spanning the Xq28-duplicated region
in the peripheral mononuclear blood cells (PMBCs) by RNA-seq. Expression
dosages of the MECP2 and IRAK1 genes showed no significant
difference between II2 and I2 (Supp. Table S6). We also detected the
mRNA of MECP2 and IRAK1 in the two females and three
normal females by RT-PCR (Figure 5). The expression dosages ofMECP2 and IRAK1 in the Xq28-duplication females were not
upregulated compared to those of the normal females.