3.2.1. ASAP species delimitation using the standard fungal barcode
A maximum of 32 species partitions were inferred from the combined ITS dataset (ITS sequences generated for this study + ITS sequences reported in Spjut et al. 2020) using ASAP, 13 of which were reprsented by the samples selected for RADseq. The majority of ASAP partitions containing at least two samples were comprised of morphologically/chemically polymorphic specimens, e.g., specimens identified as different species (Fig. 2). The ASAP partitions inferred from the standard fungal barcode marker largely coincided with reciprocally monophyletic clades inferred for RADseq data in the SVDQuartets+PAUP* and ML topologies (Fig. 2). Specimen sl16849BF (Niebla “sp. nov.”) was inferred as a distinct species partition in ASAP but was combined with another partition comprising the remainder of the specimens in the same clade for all subsequent analyses. This specimen was morphologically similar to N. versiforma but contains salazinic acid, rather than divaricatic acid (with triterpenes), and represents a putative undescribed species. Specimens sl16775 & sl16853BF, both representing isidiate Niebla specimens (Fig. 1E &K) and collected from the same locality, were inferred as separate ASAP partitions but were combined for all subsequent analyses tentatively identified as N.aff. isidiosa .