3.2.1. ASAP species delimitation using the standard fungal
barcode
A maximum of 32 species partitions were inferred from the combined ITS
dataset (ITS sequences generated for this study + ITS sequences reported
in Spjut et al. 2020) using ASAP, 13 of which were reprsented by the
samples selected for RADseq. The majority of ASAP partitions containing
at least two samples were comprised of morphologically/chemically
polymorphic specimens, e.g., specimens identified as different species
(Fig. 2). The ASAP partitions inferred from the standard fungal barcode
marker largely coincided with reciprocally monophyletic clades inferred
for RADseq data in the SVDQuartets+PAUP* and ML topologies (Fig. 2).
Specimen sl16849BF (Niebla “sp. nov.”) was inferred as a
distinct species partition in ASAP but was combined with another
partition comprising the remainder of the specimens in the same clade
for all subsequent analyses. This specimen was morphologically similar
to N. versiforma but contains salazinic acid, rather than
divaricatic acid (with triterpenes), and represents a putative
undescribed species. Specimens sl16775 & sl16853BF, both representing
isidiate Niebla specimens (Fig. 1E &K) and collected from the
same locality, were inferred as separate ASAP partitions but were
combined for all subsequent analyses tentatively identified as N.aff. isidiosa .