Extractions of specimens for RADseq were done using ZR Fungal/Bacterial DNA MiniPrep Kit (Zymo Research, Irvine, CA, USA) as previously described (Grewe et al., 2017). We amplified the ITS region (ITS1, 5.8S & ITS2) using primers ITS1 (Gardes and Bruns 1993) with ITS4 (White et al. 1990). Polymerase chain reaction (PCR) amplifications were performed using Ready-To-Go PCR Beads (GE Healthcare, Pittsburgh, PA, United States), with cycling parameters following a 66–56°C touchdown reaction (Lindblom and Ekman 2006). PCR products were visualized on 1% agarose gel and enzymatically cleaned using ExoSAP-IT Express (USB, Cleveland, OH, United States). Complementary strands were sequenced using the same primers used for amplifications, and sequencing reactions were performed using BigDye 3.1 (Applied Biosystems, Foster City, CA, United States). Products were run on an ABI 3730 automated sequencer (Applied Biosystems) at the DNA Sequencing Center at Brigham Young University, Provo, UT, United States.