2.6 | Proteases, protease inhibitors (PI) and Excretory/secretory proteins (EPs)
Proteases and PIs were annotated using MEROPS batch-BLAST(Rawlings & Morton, 2008) against MEROPS (E<1e-10). The conventional secretion proteins and non-conventional secretion proteins were identified. SignalP-5.0 Server (http://www.cbs.dtu.dk/services/SignalP/) and TMHMM Server v2.0 (http://www.cbs.dtu.dk/services/TMHMM/) were used to predict the signal peptides and transmembrane regions of proteins, respectively. The proteins with NNscore > 0.9 were selected as non-conventional protein secretion in the results of SecretomeP 2.0 Server (http://www.cbs.dtu.dk/services/SecretomeP/) identification.