Ischemia-Modified Albumin and Plasma Albumin Levels
We measured Ischemia-Modified Albumin (IMA) levels by using cobalt binding test developed by Bar- Or et al. [13]. Briefly, this method is based on the binding of cobalt with dithiothreitol (DTT) and measuring spectrophotometrically at 480 nm by adding a known amount of cobalt into the serum. The results were expressed as absorbance units (ABSU) (Abbot Architect C-8000). Plasma albumin levels were measured in autoanalyzer by using bromecresol green method (Architect Plus, C-8000, Abbott, USA).