PBAE synthesis
1,4-Butanediol diacrylate (B4), 3-amino-1-propanol (S3),
5-amino-1-pentanol (S5), 1-(3-Aminopropyl)-4-methylpiperazine (E7) (Alfa
Aesar), 2-(3-Aminopropylamino)ethanol (E6), 1-(2-Aminoethyl)piperazine
(E39) (Sigma Aldrich), 1,5-Pentanediol diacrylate (B5) (SantaCruz
Biotechnology), and 4-amino-1-butanol (S4) (Fisher Scientific) were
purchased and stored as directed. Base monomer (B4 or B5) was mixed with
sidechain monomer (S3, S4, or S5) at a 1.08:1 ratio and the reaction was
allowed to proceed for 24 h with stirring at 85°C. The neat acrylate
terminated B-S polymer was then resuspended in THF (final concentration
200 mg/mL) and combined with 0.5M end-capping monomer (E6, E7, or E39)
for 2 h with stirring at room temperature. The resulting polymer was
precipitated twice in diethyl ether and dried under vacuum for 48 h,
then resuspended to 100 mg/mL in DMSO and stored at -20°C in desiccant.