B cells stimulation
B cells were cultured in 96-well plates at 1 ×106 cells/ml in 100 μl of culture medium alone or
supplemented with various cytokines and stimuli: 1 μg/ml anti-CD40 (R&D
Systems), anti-IgM (Jackson ImmunoResearch Laboratories), 50 ng/ml IL-4
(R&D Systems), and various concentrations of PFI-1 (R&D Systems).
For human plasmablast differentiation, B cells were stimulated with
anti-IgM (5μg/ml), rCD40L (1μg/ml), CpG ODN2006 (5μM), rIL-10 (50
ng/ml), and rIL-15 (10 ng/ml).