Isolation of mouse splenocytes, lymphocytes and peripheral blood mononuclear cells
Mice were sacrificed under anaesthesia. The spleens and lymphonodus were removed and dissociated in RPMI-1640 medium (Invitrogen, Carlsbad, CA). The cell suspension was passed through a 70 μm strainer, and cells were collected by centrifugation at 300 g for 5 minutes. PBMCs were harvested by Ficoll density gradient centrifugation.