Establishment of B cell-specific Brd4-deficient mice
Floxed Brd4 mice (Brd4flox/WT) were generated by CRISPR/Cas-mediated genome engineering technology (H. Yang, Wang et al., 2014), which was supported by the Nanjing Biomedical Research Institute of Nanjing University (Nanjing, China). The Brd4 gene was subjected to editing in embryos to generate mice carrying conditional alleles. LoxP sites for Cre-mediated recombination were inserted 5′ and 3′ into the fifth coding exon. B cell-specific Brd4-deficient mice were generated through appropriate breeding of Brd4flox/WT mice with CD19-Cre mice (Stock No: 006785, The Jackson Laboratory) and maintained on a C57BL/6 background. Brd4flox/floxCD19-Cre-/-(control) and Brd4flox/floxCD19-Cre+/-(knockout) mice were born at the expected Mendelian ratio, and these mice did not show any gross defects in survival.