Pristane-induced murine lupus nephritis model
A single intraperitoneal injection of 0.5 mL pristane or PBS using a 1 mL syringe was applied to 6- to 8-week-old female Brd4flox/floxCD19-Cre +/- mice or Brd4flox/floxCD19-Cre -/- mice. The mice were housed under specific pathogen-free conditions under a 12-hour light/dark cycle. Serum and urine samples were collected monthly. Spleen, kidney, lymph node, and PBMCs were obtained at 6 months after pristane injection. Kidney histopathologic changes were determined by H&E staining. No mice died during the course of the experiments.