Establishment of B cell-specific Brd4-deficient mice
Floxed Brd4 mice (Brd4flox/WT) were generated by
CRISPR/Cas-mediated genome engineering technology (H. Yang, Wang et al.,
2014), which was supported by the Nanjing Biomedical Research Institute
of Nanjing University (Nanjing, China). The Brd4 gene was subjected to
editing in embryos to generate mice carrying conditional alleles. LoxP
sites for Cre-mediated recombination were inserted 5′ and 3′ into the
fifth coding exon. B cell-specific Brd4-deficient mice were generated
through appropriate breeding of Brd4flox/WT mice with
CD19-Cre mice (Stock No: 006785, The Jackson Laboratory) and maintained
on a C57BL/6 background.
Brd4flox/floxCD19-Cre-/-(control)
and Brd4flox/floxCD19-Cre+/-(knockout) mice were born at the expected Mendelian ratio, and these
mice did not show any gross defects in survival.