Pregnancy and clinical outcomes
Table 1 presents the effects of different post-thawed culture periods on
pregnancy and clinical outcomes. The results indicated that the
post-thawed culture period had significant effects on pregnancy and
clinical outcomes. In total age group, IR, CPR, MPR and LBR of L group
were observed to be higher than that of S group (P /CI =
0.002/1.034-1.162, 0.027/1.011-1.194, 0.028/1.014-1.255 and
0.001/1.054-1.245 respectively), while EPR was significant high in short
culture group (P /CI = 0.029/1.043-1.983) and AR was no
significant difference between the two groups (P /CI =
0.172/0.962-1.260). In the < 35 year groups, IR, CPR and LBR
were significant high in the L group compared with these in the S group
(P /CI = 0.001/1.061-1.233, 0.004/1.056-1.332, 0.000/1.118-1.396
respectively), while AR and EPR were observed to be higher in the S
group than these in the L group (P /CI = 0.036/1.015-1.472,
0.002/1.272-2.864 respectively). Moreover, MPR was high of L group than
that of S group (P /CI = 0.071/0.990-1.280). In the 35-39 age
group, IR, CPR, MPR, EPR and AR were similar between groups L and S,
while LBR was observed to be higher in the L group than these in the S
group (P /CI = 0.039/1.010-1.387). In the > 39 age
group, there was no significant difference on IR, CPR, MPR, AR, EPR and
LBR between S and L groups. Table 1 also displays the effect of female
age on pregnancy and clinical outcomes. The results indicated that IR,
CPR, MPR and LBR decreased significantly and AR increased significantly
(P values were all less than 0.001) with age in the S and L
groups, while EPR had not significant difference with age.
Table 2 shows the effect of blastomere growth number after long culture
period of thawed D3 embryo on pregnancy and clinical outcomes. The
results indicated that blastomere growth number had a significant effect
on IR, CPR and LBR in all age groups (P values were all less than
0.001), and the faster the embryo developed, the better outcome was
found. MPR significantly increased with the increase of blastomere
growth number in < 35, 35-39 and total age groups (P =0.000, 0.001 and 0.000 respectively), while MPR was similar in
> 39 age group
(P = 0.235). AR was similar
in < 35 and > 39 age groups (P = 0.353 and
0.948) and the rates of ≥ 2 blastomeres groups significantly decreased
in 35-39 and total age groups (P = 0.028 and 0.050). Moreover, no
significant differences were found in EPR among the four age groups. The
IR, CPR and LBR of same blastomere growth number groups decreased
significantly and AR increased significantly with age (P values
were all less than 0.001).
A logistic regression model for predicting live birth is given in Table
3. Variables that were statistically significant in Table S1, or those
that were deemed to be clinically significant, were included in order to
build the model. This model indicated that the most significant
predictors for live birth were female age, type of infertility and
culture period (D3/D4). This model shows that the most significant
predictors for live birth were female age,
secondary
infertility
and long post-thawed culture period (P /CI = 0.00/0.89-0.91,
0.00/1.06-1.28 and 0.00/1.11-1.32 respectively).