3.4. Statistical analyses comparing CBH with metabarcoding
The ten samples chosen for this comparative study were collected at five different sites, including two different ecosystems, seamounts (in the Atlantic Ocean and the Mediterranean) and hydrothermal vents (inactive site, characteristic of deep bathyal sediment systems), to test different biological communities. Based on multidimensional analysis from Bray Curtis (based on the number of sequences) distances of CBH-short and MTB methods for prokaryote communities, the methods clearly appeared to be the dominant driver of differentiation (Appendix 6A). Mantel test showed that all three analyses were significantly different from each other (p<0.01). In contrast, for 18S detection, clusters were segregated per location of origin rather than method (Appendix 6B). Mantel test showed no significant difference (p=0.02), suggesting less technical bias in the community description of eukaryotes.