Abstract
Objective:Although specific
anti-phospholipids antibodies (aPLs) have been used in the diagnosis of
the antiphospholipid syndrome (APS) for years, new biomarkers are
required to increase its diagnostic as well as risk-predictive power.
This study aimed to explore the value of several extra-criteria aPLs in
a Chinese cohort.
Methods: A total of 312 patients including 100 patients
diagnosed with primary APS, 51 with APS secondary to SLE, 71 with SLE,
and 90 health controls were recruited. Serum anticardiolipin (aCL)
IgG/IgM/IgA, anti-β2-glycoprotein I (aβ2GPI) IgG/IgM/IgA,
anti-phosphatidylserine/prothrombin antibodies (aPS/PT) IgG/IgM,
anti-annexin A5 antibodies (aAnxV) IgG/IgM were tested using ELISA kits.
Results: Totally 30.46% and 6.62% of patients with APS were
positive for aCL or aβ2GPI IgA respectively, while 39.07% and 24.50%
were positive for aAnxV or aPS/PT for at least one antibodies (IgG or
IgM). The addition test of aCL IgA and aAnxV IgM assist in identifying
seronegative APS patients, and IgG aANxV was linked with stroke.
Conclusion: Detection of aCL IgA, aβ2GPI IgA, aAnxV IgG/M, and
aPS/PT IgG/M as biomarker provide additive value in APS diagnosis, and
would help in risk prediction for APS patients in medical practice.
Keywords antiphospholipid syndrome, antiphospholipid
antibodies, immunoglobulin A, anti-phosphatidylserine/prothrombin,
anti-annexin V