Laboratory tests
IgG, IgM, and IgA isotypes of aCL and aβ2GPI, IgG and IgM isotypes of
aPS/PT and aAnxV were analyzed with AESKULISA® ELISA Test Kits provided
by Aesku. Diagnostics GmbH & Co. KG (Wendelsheim, Germany). Cut-off
value was defined as 18 U/mL as recommended by the manufacturer. Lupus
anticoagulant was detected and evaluated at the Key Laboratory according
to the ISTH recommendations measuring Dilute Russell viper venom time
(dRVVT)/activated partial thromboplastin time (>1.20 as
positive).