Laboratory tests
IgG, IgM, and IgA isotypes of aCL and aβ2GPI, IgG and IgM isotypes of aPS/PT and aAnxV were analyzed with AESKULISA® ELISA Test Kits provided by Aesku. Diagnostics GmbH & Co. KG (Wendelsheim, Germany). Cut-off value was defined as 18 U/mL as recommended by the manufacturer. Lupus anticoagulant was detected and evaluated at the Key Laboratory according to the ISTH recommendations measuring Dilute Russell viper venom time (dRVVT)/activated partial thromboplastin time (>1.20 as positive).