Enzyme-linked immunosorbent assay (ELISA)
PB was collected from patients, HCs and mice in heparin sodium to
centrifuge plasma. The levels of IFN-γ (Catalog #EK180HS), IL-4
(Catalog #EK104HS), IL-10 (Catalog #EK110HS) and IL-17A (Catalog
#EK117HS) were determined by Human High Sensitivity ELISA kit
(MultiSciences Biotech Co., Ltd., Hangzhou, China), and the leves of
IL-1β (Catalog #EK201BHS), IL-6 (Catalog #EK206HS), TNF-α (Catalog
#EK282HS), IL-10 (Catalog #EK210/3), IL-17A (Catalog #EK217HS) were
detected by Mouse High Sensitivity ELISA kits or Mouse ELISA kits
(MultiSciences Biotech Co., Ltd., Hangzhou, China). All ELISA procedures
subjected to the manufacturer’s instructions.