Intracellular staining and flow cytometry
As previously described[25,26], splenocytes and
PBMCs from CIA mice were washed with PBS. For the analysis of Treg
cells, cells were stained with anti-CD4-FITC (BD Bioscience),
anti-CD25-PE-CY7 (BD Bioscience) and anti-Foxp3-PE (BD Bioscience). For
analysis and gating, we set up auxiliary staining groups including no
staining, single staining, isotype staining. Finally, data were acquired
on flow cytometry (Beckman FC-500, USA).