Enzyme-linked immunosorbent assay (ELISA)
PB was collected from patients, HCs and mice in heparin sodium to centrifuge plasma. The levels of IFN-γ (Catalog #EK180HS), IL-4 (Catalog #EK104HS), IL-10 (Catalog #EK110HS) and IL-17A (Catalog #EK117HS) were determined by Human High Sensitivity ELISA kit (MultiSciences Biotech Co., Ltd., Hangzhou, China), and the leves of IL-1β (Catalog #EK201BHS), IL-6 (Catalog #EK206HS), TNF-α (Catalog #EK282HS), IL-10 (Catalog #EK210/3), IL-17A (Catalog #EK217HS) were detected by Mouse High Sensitivity ELISA kits or Mouse ELISA kits (MultiSciences Biotech Co., Ltd., Hangzhou, China). All ELISA procedures subjected to the manufacturer’s instructions.