Separation of peripheral blood CD4+ T cells
For human samples, peripheral blood mononuclear cells (PBMCs) were
isolated by Ficoll density gradient centrifugation (TBD sciences,
LTS1077). The separated PBMCs were washed twice with BD IMagTM buffer
and then incubated with BD IMagTM Anti-Human CD4 Particles (BD
Biosciences, San Jose, CA) on ice for 30 min. 1 mL BD IMagTM buffer
containing 107 cells were placed in the magnet for 8
min, and then CD4+T cells were collected.