4 Conclusion
In this study, the separation and purification process of γ-tocopherol
was optimized. Under the optimal conditions, the purity of γ-tocopherol
reached (98.38±0.68) %, and the yield was (93.23±0.89) %. The method
adopted in this study could obtain high-purity γ-tocopherol, and was
simple and easy to operate and more eco-friendly, which can be performed
in plant-scale and provide certain theoretical support for the
production of γ-tocopherol. Furthermore, we compared the antioxidant
activities of α-, γ- and δ-tocopherol and found that γ-tocopherol had
the best antioxidant activities among these three tocopherol monomers,
our results could provide data supports for the further application of
tocopherols.