3.5.1 TLC analysis of purified γ-tocopherol
TLC was used to analyze the components of the purified γ-tocopherol, and compared it with the γ-tocopherol standard substances (Fig. 2b). Results showed that non-γ-tocopherol (α- and δ-tocopherol) and most of the impurities in the mixed tocopherols samples have been removed after one simple column chromatographic separation. And the spots on silica gel plate of purified γ-tocopherol was fewer than that of γ-tocopherol standard, which indicated that the purity of our purified γ-tocopherol was much higher than that of γ-tocopherol standard.