4 Conclusion
In this study, the separation and purification process of γ-tocopherol was optimized. Under the optimal conditions, the purity of γ-tocopherol reached (98.38±0.68) %, and the yield was (93.23±0.89) %. The method adopted in this study could obtain high-purity γ-tocopherol, and was simple and easy to operate and more eco-friendly, which can be performed in plant-scale and provide certain theoretical support for the production of γ-tocopherol. Furthermore, we compared the antioxidant activities of α-, γ- and δ-tocopherol and found that γ-tocopherol had the best antioxidant activities among these three tocopherol monomers, our results could provide data supports for the further application of tocopherols.