Determination of physiological indices
The MDA (malondialdehyde) content in leaves of Arabidopsis plants were
performed as described previously (Breuil & Saddler, 1985; Shabala,
Shabala, Martynenko, Babourina, & Newman, 1998; Sofo, Dichio,
Xiloyannis, & Masia, 2004). Relative electrolyte leakage (%) in leaves
of Arabidopsis plants was determined according to the (Dionisio-Sese &
Tobita, 1998) method. The total phosphorus content was determined by
using Total Phosphorus Assay Kit (Yoyobio, Shanghai, China). The
inorganic phosphorus content was determined by using Inorganic
Phosphorus Assay Kit. The content of starch and soluble sugar were
determined by using Starch Kit and Soluble Sugar Kit. The enzyme
activities of amylases, SOD, POD and CAT were determined by using
Amylases Kit, SOD Kit, POD Kit and CAT Kit. All of the above unmarked
Kits are produced from Comin (Suzhou, China).