Histochemical staining and determination of ROS content
The content of H2O2 and O2.- in leaves of Arabidopsis plants could be observed by histochemical staining with DAB and NBT. Specific steps of histochemical staining have been described by predecessors (Wu et al. 2017). The difference to previous study is PH, the PH of NBT and DAB solution were individually adjusted to 7.5 and 5.8. The quantitative content of H2O2and O2.-in Arabidopsis thaliana were determined by spectroscopic method using Hydrogen peroxide (H2O2) content Kit and Superoxide anion (O2.-) Kit (Comin).