Histochemical staining and determination of ROS content
The content of H2O2 and
O2.- in leaves of Arabidopsis plants
could be observed by histochemical staining with DAB and NBT. Specific
steps of histochemical staining have been described by predecessors (Wu
et al. 2017). The difference to previous study is PH, the PH of NBT and
DAB solution were individually adjusted to 7.5 and 5.8. The quantitative
content of
H2O2and
O2.-in Arabidopsis thaliana were determined by spectroscopic method
using Hydrogen peroxide (H2O2) content
Kit and Superoxide anion (O2.-) Kit
(Comin).