Pharmacological and cell culture reagents
Lipopolysaccharide (LPS; Escherichia coli strain O26:B6; #L8274), 2-deoxy-D-glucose (2-DG; #D8375), Trolox (TROL; #238813), dexamethasone (DEX; #D4902) and doxycycline hyclate (DOX; #D9891) were obtained from Sigma Aldrich (L’Isle d’Abeau Chesnes, France). 4-Dedimethylaminosancycline (COL-3; #HY-13648) was purchased from MedChemExpress (Monmouth Junction, NJ). Apocynin (APO; #4663) was purchased from R&D Systems Europe (Lille, France). Dulbecco’s modified Eagle’s medium (DMEM) and the Trypsin (0.05%)-EDTA solution used to produce subcultures were obtained from ThermoFisher Scientific (Saint Aubin, France). Fetal calf serum (FCS) was provided by Biowest LLC (Eurobio, Les Ulis, France). Deoxy-D-glucose 2-[1,2-3H (N)]- (NET549A) was purchased from Perkin Elmer (Courtaboeuf, France).