Quantification of intracellular ROS levels
Intracellular ROS levels were measured using the membrane-permeable
CellROX Deep Red reagent (Invitrogen Life Technologies), as described
before [33]. Signal acquisition was performed an Arrayscan XTI
automated workstation fitted with a 20x objective (Thermo Scientific,
Courtaboeuf, France). Cellular fluorescence intensity levels were
estimated with the HCStudio software [30].