Assessment of [3H]-2-DG uptake
The uptake of [3H]-2-DG was measured based on a
protocol previously described [32]. Briefly, after termination of
test treatments, microglial cell cultures were incubated at
37oC for 30 min in PBS containing 1 μCi
[3H]-2-DG. 2-DG accumulation was terminated by
washing the cultures with ice-cold PBS and the radioactivity
incorporated by the cells recovered by scraping the cultures in
distilled water containing 1% Triton-X. The radioactivity accumulated
by microglial cells was quantified with a Tri-Carb 4910TR liquid
scintillation counter (Villebon sur Yvette, France). Blank values were
obtained by applying 50 mM glucose to control cultures.