Evolutionary rate of salmonids
Studies of mutation frequencies over time have shown that species vary in their evolutionary rates. Thus, the evolutionary rate of B. l. tsinlingensis and other salmonids may be quite different from other fishes. The evolutionary rate of salmonids was quite similar and much slower compared with other outgroup species. However, the evolutionary rate of B. l. tsinlingensis was the fastest of all previously published evolutionary rates for salmonids (Figure 4D; Table S19-S20 ). These results indicate shown that after Ss4R, all of the salmonids occupied a stable environment and thus had a much slower evolutionary rate; however,B. l. tsinlingensis is still threatened and requires protection.