WGD assessment
The distribution of synonymous substitutions per site (Ks) within
paralogs was used to examine the most recent WGD event in salmonids. The
protein sequences of all 11 species were aligned with BLAST v2.9.0
(e-value 1e-10) (Altschul et al.1990). When one gene and another gene were mutual best hits (excluding
hits to themselves), they were identified as a paralog genes. Ks was
calculated using the KaKs_calculator v2.0
(Wang et al. 2010) for each
paralog. For comparison, we also plotted the Ks distribution betweenB. l. tsinlingensis and other species.