Genome data generation
The muscle, heart, kidney, liver, and spleen were obtained from one
adult, and all samples were stored in -80℃ for DNA and RNA extraction.
Genomic DNA was extracted using a Qiagen DNeasy Blood & Tissue Kit. The
quality of the extracted DNA was determined using an Agilent 2100
bioanalyzer (Agilent Technologies). The RNA of these tissues/organs was
extracted using Trizol, and the quality of the extracted RNA was also
assessed by an Agilent 2100 bioanalyzer (Agilent Technologies) and
evaluated on a 1.5% agarose gel stained. Both DNA and RNA were used for
the construction of different libraries and further sequencing. Genomic
DNA was sequenced on a NovaSeq platform (Illumina) with a short-insert
pair-end mode and Oxford Nanopore platform, respectively. RNA sequencing
and Hi-C sequencing were both conducted on the NovaSeq platform
(Illumina).