Genome quality evaluation
After the genome assembly process, several genome quality evaluation
methods were used. We used BUSCO (v2.0) software
(Simao et al. 2015) to estimate
the percentage of conserved orthologs in the assembled genome. The
conserved gene sets of Eukaryota and Metazoa were used as a database and
the complete BUSCO genes, fragment genes, and missing genes were
detected. BWA (Li& Durbin 2009) and BLAT
(Kent 2002) were used for the mapping
ratio of NGS data and de novo assembled transcripts,
respectively.