The realization of the enormous potential of stem cells requires
development of efficient bioprocesses and optimization drawing drawn
from mechanobiological considerations. Here, we emphasize the importance
of mechanotransduction as one of the governing principles of stem cell
bioprocesses, underscoring the need to further explore the behavioral
mechanisms involved in sensing mechanical cues and coordinating
transcriptional responses. We identify the sources of the intrinsic,
extrinsic, and external noise in bioprocess under uncertainty, and
discuss criteria and indicators that might assess and predict
cell-to-cell variability resulting from environmental fluctuations.
Specifically, we propose a conceptual framework to explain the impact of
mechanical forces within cellular environment and identify key cell
state determinants in bioprocess and discuss their implementation
challenges.