Supplemental Material
Figure S1 The workflow of the methods of this study: (1)
selection of samples and collection of patients’ clinical data; (2)
Analysis of COX-2 expression in FFPE samples; (3) genome analysis by
a-CGH; (4) gene data extraction; (5) systems biology approach
Figure S2 MYCN amplification status, as determined by
FISH analysis. A) Representative images of FISH analysis of NB samples
hybridized with the MYCN probe. Samples classified as in (a) and
(b) were considered as non-amplified MYCN (NAMN). Samples with
more than ten copies were considered as amplified MYCN (AMN). B)
Percentage of NB cases (n = 84) in each MYCN amplification group
Figure S3 Representation of the interconnection of genes of
higher relevance in the network as described previously. It shows the
mutual association of inflammation genes and COX-2 resulting in the
activation of metalloproteinase-3
Table S1 Chr11q-deleted locus in pre- and post-CT samples from
three patients
Table S2 RNA genes in Chr11q deleted cytobands.
Table S3 miRNAs pathways and targets.
Table S4 Network Analysis. Chr11q protein-coding genes,
inflammation-related genes, and miRNA target genes; CentiScape Analysis;
MCODE Analysis; Biological Process.
Table S5 Gene Ontologies (GO) Analysis.