Figures captions
Figure 1 IHC analysis of COX-2 expression in NB tumors. Mean percentage of the stained area of COX-2 in NB/GNB. A) Unpaired (n = 77) pre/post-CT samples. Statistical differences between pre/post groups were determined by t-test, using GraphPad Prism v.8. B) Representative IHC image of COX-2 expression in a pre- and post-chemotherapy paired sample (Patient 42; 20X magnification)
Figure 2 Cytobands with copy number alterations (CNA) in paired NB samples pre-and post-chemotherapy. A) Median of CNAs per case. B) List of CNAs affecting at least five (50%) cases, including those of prognostic importance (*) and those involved in the COX-2 pathway or correlated with COX-2 expression (#), in pre- and post-treatment samples from each patient. Light grey squares indicate losses, and dark grey squares, gains. C) Prostaglandin 2 (PGE2) pathway showing the main enzymes and the chromosome location of the genes involved
Figure 3 Interval of loss or deletions in Ch11q from three patients’ samples classified as Group 2 (NAMN/11q-). A) Start and stop positions of the affected cytobands. Below: Chromosome intervals affected by CNAs for each patient in Group 2 B) Venn diagram presenting the relationships of genes located at the cytobands affected by the copy number alterations
Figure 4 PPI network topological analysis. A) Left, complete network showing the 468 gene interconnections. Nodes in blue are from the Chr11q protein-coding genes region, in yellow genes from inflammation pathways, and green Chr11q-miRNAs targets. The PTGS2gene is marked in purple. Right, Venn diagram representing CentiScape centrality analysis (degree, betweenness, eigenvector). Below, biological processes (gene ontologies - BinGo), with the highlights of five of them: steroids, cell adhesion, transcription, DNA repair, and inflammation. The adhesion process was present in all the categories. B) Clustering analysis by MCode showing the five clusters identified. Clusters 1, 3, and 5 presented several genes in common (arrows); cluster 3 was the only one composed of the PTGS2 gene. Genes marked with * are HBS genes. The common genes for Clusters 1, 3, and 5 are in the center