Genetic analysis
DNA was isolated from peripheral blood samples using the MagNa Pure Compact Nucleic Acid Isolation Kit I (Roche) according to the manufacturer’s instructions. A next generation sequencing (NGS) panel including genes related to CMC (STAT1, TRAF3IP2, CARD9, IL17F, IL17RA, and IL17RC ), was performed using an AmpliSeq strategy on the Ion Torrent PGM platform. Primary sequence data analysis was performed with Torrent server 5.1 software. Single-nucleotide polymorphisms were denoted as proposed by the Torrent Variant Caller v5.12.0.40 and analyzed by Ion Reporter Sofware v5.12. Identified specific mutations were validated by Sanger sequencing using specific primers amplifying exon 6 of TRAF3IP2 gene and exon 17 ofSTAT1 .