2.7. Statistical analysis
GraphPad Prism software, version 8 (GraphPad Software Inc., La Jolla, USA) was used for plotting graphs and statistical analysis. All values were expressed as mean ± standard deviation (SD). Statistical analysis was performed using Mann–Whitney U test for Pepscan analysis and Wilcoxon test for comparing reactivity against reduced and native target proteins and depletion assays analysis. Differences were considered to be significant at P<0.05(*), P<0.01(**), P<0.001(***), and P<0.0001(****).