2.7. Statistical analysis
GraphPad Prism software, version 8 (GraphPad Software Inc., La Jolla,
USA) was used for plotting graphs and statistical analysis. All values
were expressed as mean ± standard deviation (SD). Statistical analysis
was performed using Mann–Whitney U test for Pepscan analysis and
Wilcoxon test for comparing reactivity against reduced and native target
proteins and depletion assays analysis. Differences were considered to
be significant at P<0.05(*), P<0.01(**),
P<0.001(***), and P<0.0001(****).