3.3 Identification of the recombinant ZnT8/GAD65 protein
expressed in Sf9 cells by indirect ELISA
Identification of the ZnT8 and GAD65 domains in the recombinant chimera
was evaluated by indirect ELISA using ZnT8/GAD65 as the immobilized
antigen that interacted with polyclonal rabbit ZnT8 antibodies and
monoclonal mouse GAD65 antibodies. As it is shown in Figure 4, both
domains were correctly recognized by their specific antibodies,
demonstrated by the significant difference of the signals obtained with
the specific antibodies with respect to the normal controls. This
encouraged us to go ahead with the design of a bridge ELISA for the
detection of autoantibodies in individuals at risk or diabetic patients
with doubtful classification.