2.5.2 Biotinylation of the chimera ZnT8/GAD65
Biotinylation of the purified chimera was done as we have previously
described (Trabucchi et al., 2019, Trabucchi et al, 2022). Two hundred
eighty micrograms (280 ug) of desalted protein were incubated with
excess of sulfo-NHS-biotin (Pierce Biotechnology), and free biotin was
then removed.
ZnT8/GAD65-biotin was stored until use at -20 °C with the addition of
50% v/v glycerol, Tween 20 and protease inhibitors.