C1
Due to a paucity of initial hits (17 total genera), the genus-level
filter was removed for all phyla to increase resolution of the domain
phylogeny. A preliminary tree (Fig. S2) was expanded to identify
outgroup sequences by including hits with E ≤ 10-10.
The resulting tree was rooted using minimal ancestor deviation (MAD)
rooting (Tria, Landan and Dagan, 2017).