Genetic Analysis
Blood samples were drawn directly into PAXgene tubes (Qiagen Inc,
Valencia, Calif) and immediately frozen and stored at -80 degrees
Celsius for mRNA stabilization and extraction, per the manufacturer’s
recommendation.
Human transcriptome profiling assay were done with Applied Biosystems™
Clariom™ D Pico Assay, human (ThermoFisher Scientific) according to the
manufacturer’s protocol in Brown University Genomics Core Facility for
use on the GeneChip™ 3000 instrument system. Transcriptome Analysis
Console (TAC) software was used to analyze and visualize global
expression patterns of genes, exons, pathways, and alternative splicing
events.