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\begin{document}

\title{Macroparticle-enhanced cultivation of \emph{Lentzea aerocolonigenes}:
Variation of mechanical stress and combination with lecithin
supplementation for a significantly increased rebeccamycin production}



\author[1]{Kathrin Schrinner}%
\author[2]{Marcel Schrader}%
\author[2]{Jana Niebusch}%
\author[2]{Kristin Althof}%
\author[2]{Friederike Schwarzer}%
\author[2]{Paul-Frederik Nowka}%
\author[2]{Anna Dinius}%
\author[2]{Arno Kwade}%
\author[1]{Rainer Krull}%
\affil[1]{TU Braunschweig}%
\affil[2]{Technische Universität Braunschweig}%


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  \date{May 22, 2024}


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\begin{abstract}
The actinomycete \emph{Lentzea aerocolonigenes} produces the antitumor
antibiotic rebeccamycin. In previous studies the rebeccamycin production
was significantly increased by the addition of glass beads during
cultivation in different diameters between 0.5 -- 2 mm and the induced
mechanical stress by the glass beads was proposed to be responsible for
the increased production. Thus, this study was conducted to be a
systematic investigation of different parameters for macroparticle
addition, such as bead diameter, concentration and density (glass and
ceramic) as well as shaking frequency, for a better understanding of the
particle induced stress on L. aerocolonigenes. The induced stress for
optimal rebeccamycin production can be estimated by a combination of
stress energy and stress frequency. In addition, the
macroparticle-enhanced cultivation of L. aerocolonigenes was combined
with soy lecithin addition to further increase the rebeccamycin
concentration. With 100 g L\textsuperscript{-1} glass beads in a
diameter of 969 \selectlanguage{greek}µ\selectlanguage{english}m and 5 g L\textsuperscript{-1} soy lecithin a
concentration of 388 mg L 1 rebeccamycin was reached after 10 days of
cultivation, which corresponds to the highest rebeccamycin
concentrations achieved in shake flask cultivations of \emph{L.
aerocolonigenes} stated in literature so far.%
\end{abstract}%



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\verb`Schrinner et al - Macroparticle enhanced cultivation - Biotechnol Bioeng_2021-06-03.docx`  available at \url{https://authorea.com/users/419491/articles/525975-macroparticle-enhanced-cultivation-of-lentzea-aerocolonigenes-variation-of-mechanical-stress-and-combination-with-lecithin-supplementation-for-a-significantly-increased-rebeccamycin-production}\selectlanguage{english}
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