Figure 4. Compound 270 ameliorates the LPS-induced inflammatory response in bone marrow derived macrophages (BMDMs)
(A) BMDMs were pretreated with 270 for 4 h before LPS (100 ng/mL) treatment for 30 min. Protein was isolated from the BMDMs and inflammation signaling proteins were assessed by western blot. (B) The protein levels shown in (A) were quantified by densitometry analysis. (C) BMDMs were treated with LPS (100 ng/ml) alone or together with 270 for 24 h. The concentration of TNF-α, IL-1β and MCP 1 in the medium were examined by ELISA (n = 3). (D) BMDMs were treated with LPS (100 ng/ml) alone or together with 270 for 24 h. Total RNA was extracted from BMDMs and the relative mRNA level of each pro-inflammatory gene was detected by use of RT-PCR and normalized by GAPDH. The data are presented as the means ± SEM. All experiments were performed in three replicates. Two-tailed Student’s t-test. *P < 0.05, **P <0.01, ***P < 0.001 versus LPS group.
Figure 5