2.10 Biochemical tests and detection of cytokines
The concentrations of TNF-α, IL-1β, IL-6 and MCP 1 in mice plasma and BMDMs supernatants were detected using ELISA kits, according to the manufacturer’s instructions. To evaluate kidney function, urea nitrogen (BUN) and creatinine (Crea) levels in mice plasma were estimated using commercially available kits (Xinjiankangcheng), and performed following the manufacturer’s protocol.