PCA and Heatmap analyses of CAZymes and lignocellulolytic genes
Results of the PCA of CAZymes and lignocellulolytic genes showed a clear separation of WR and BR fungi along PCA1 (Figures 2a and S3a). All the fungi in Auriscalpium and Strobilurus cluster together with other WR fungi, consistent with their potent lignin-decomposing ability (Figures 2a and S3a). The heatmap analyses showed that there are unique enzymes within each of the 22 fungi analyzed, which likely reflect the diversity of their preferred substrates (Figures 2c and S3b). In the separate analyses of the two genera, it is apparent that fungi in the two genera showed complementary profiles of carbohydrate-active enzymes (CAZymes) (Figures 3a and 4g–h). The results clearly illustrate that there are remarkable differences in the overall pattern of genes between two different genera. At the same time, it demonstrates that the type and number of these CAZymes inAuriscalpium and Strobilurus are different among different fungi, which may partly explain the cause of the substrate specificity (Figure S4a–b). The results of the lignocellulolytic genes are overall consistent with the results of CAZymes (Figure S4c–d). These lignocellulolytic genes are also specific among genera, and there are great differences in the type and number of enzymes among different genera, and each has a set of different enzyme system (Figures 4g–h and S4c–d).