Identification of CAZymes and lignocellulolytic genes in fungi
of Auriscalpium and Strobilurus
The number of CAZymes in A . vulgare , A .microsporum , A . orientale , S .stephanocystis , S . luchuensis , S .pachycystidiatus and S . orientalis were 450, 425,
464, 532, 542, 583 and 621, respectively (Figure 2b; Table S1). Our
statistical analyses revealed that the average number of CAZymes inAuriscalpium fungi is significantly lower than that inStrobilurus such as AA5, CBM18, CBM67, CE12, CE16, CE4, CE8,
GH105, GH12, GH127, GH128, GH13, GH135, GH16, GH17, GH18, GH27, GH28,
GH29, GH35, GH43, GH45, GH5, GH53, GH55, GH71, GH76, GH93, GT1, GT15,
GT17, GT33, GT4, GT8, PL1 and PL4 (Table S3). The average number of
CAZymes in Auriscalpium fungi is significantly more than that inStrobilurus only in seven gene families including AA2, GH2, GH3,
GH15, GH31, GH109 and PL8. Similarly, in the statistical analyses, the
comparison of CAZymes between Auriscalpium and other WR fungi,
and the comparison of CAZymes between Strobilurus and other WR
fungi are shown in
Table
S4 and Table S5, respectively. The average number of CAZymes inAuriscalpium fungi is significantly more than that in other WR
fungi only in six gene families, however the average number of CAZymes
in Strobilurus fungi is significantly more than that other WR
fungi in 31 gene families (Tables S4–S5).
The number of predicted lignocellulolytic genes in A .vulgare , A . microsporum , A .orientale , S . stephanocystis , S .luchuensis , S . pachycystidiatus and S .orientalis were 112, 97, 111, 107, 106, 122 and 111, respectively
(Table S9). For the fungi growing on cones of the same pine species, the
proportions of genes encoding ligninases and hemicellulases were higher
in Auriscalpium species than those in their correspondingStrobilurus species. In contrast, the proportions of genes
encoding cellulases and pectinases were lower in Auriscalpiumthan those in the Strobilurus . For fungi living on cones ofP . armandii , A . microsporum and S .pachycystidiatus living on relatively newly fallen cones also
showed a similar pattern but the difference was less obvious than those
in other two fungal pairs. However, S . orientalis with a
preference of living on more rotten cones showed more obvious
differences in their respective enzymes compared with A .microsporum .