Figure 2. Luminescence intensity thermometry measurements of an individual lanthanide doped nanoparticle. Representative spectra taken at two temperatures. Copyright Springer Nature 2018.
Single molecule spectroscopy on nanoparticle-protein conjugate has not been reported so far. However, conjugation of proteins with nanoparticles has been proposed. For example, Figure 3 shows an image of a space-filling modeling approach to study interactions between a maltose-binding protein and a semiconductor quantum dot,19 with surface sulfur atoms in teal and zinc atoms in pink. The red shell shows the estimated outer radius of the dihydrolipoic acid ligand. The side chain of Lys-370 is shown in blue.