Flow cytometry
Peripheral blood samples were collected and 100 μl was absorbed and added into flow tube. Then 10μl CD45/CD4/CD8/CD3 flow antibody was added. After swirl shock, the cells were incubated for 15-20min in dark at room temperature. After incubation, add 500μl Optilyse-C, mix with swirl vibration, and incubate for 10min in dark at room temperature. Add 100μl normal saline, stir and mix, and stand for 10min at room temperature in the dark. Then, mix Flow-Count vortex oscillation for 10s, take 100μl of the mixture and the data were analysed using FlowJo software.