Flow cytometry
Peripheral blood samples were collected and 100 μl was absorbed and
added into flow tube. Then 10μl CD45/CD4/CD8/CD3 flow antibody was
added. After swirl shock, the cells were incubated for 15-20min in dark
at room temperature. After incubation, add 500μl Optilyse-C, mix with
swirl vibration, and incubate for 10min in dark at room temperature. Add
100μl normal saline, stir and mix, and stand for 10min at room
temperature in the dark. Then, mix Flow-Count vortex oscillation for
10s, take 100μl of the mixture and the data were analysed using FlowJo
software.