2.6 Quantification of extracellular metabolites
Glucose and lactate were monitored daily in this study. Briefly, 200 µL
of cell culture supernatant was aliquoted into a round bottom 96 well
microtiter plate (Corning Inc. New York, United States) which was then
run on the YSI 2950D metabolite analyser (YSI Inc., Yellow Springs,
USA).