FIGURE CAPTIONS
Figure 1: Set-up of 24-DSW scale-down model for LVV production.
Figure 2: Liquid phase mixing times for 2 L STR (A) and 24-DSW plate (B) measured using the dual indicator system for mixing time (DISMT) technique. For the 2 L STR, mixing time was characterised at stirrer speeds between 100 – 300 rpm and two different bioreactor working volumes, VL = 1.5 L and VL = 1.8 L. For the 24-DSW plate, mixing time was characterised at shaking speeds between 175 – 300 rpm (ds = 25 mm) and four different fill volumes between 2 – 5 mL. Error bars represent one standard deviation about the mean (n=5).
Figure 3: Comparison between data shown in present study with the mixing time model reported in Rodriguez et al ., 2014. The model is based on the Froude number ratio (Fr/Frc) and is used for scaling between OSR’s of different sizes and geometries.
Figure 4: Cell culture profile for cultivation of a HEK293T stable producer cell line in 24-DSW plate with 5 mL starting volume and 2 L STR at matched mixing time, tm of < 10 s. (A) Viable cell density (B) Cell viability (C) Glucose concentration (D) Lactate concentration. Error bars represent one standard deviation about the mean (n = 3); Note: For day 0 and day 2 time points in Figure 4 C and 4 D, n = 2 is reported as the data for one vessel at each time point was considered to be erroneous i.e. the measurements from the metabolite analyser were below the lower limit detection range suggesting the target metabolites were not detected in the sample.
Figure 5: pH profile for cultivation of a HEK293T stable producer cell line in the 24-DSW plate. Error bars represent one standard deviation about the mean (n = 3).
Figure 6: LVV productivity profile in 24-DSW plate with 5 mL starting volume and 2 L STR at matched mixing time,tm of ~ 9 s. The infectious titre and physical titre values were normalised relative to the 2 L STR. Error bars represent one standard deviation about the mean (n = 3). Statistical comparisons were done using t-test where ‘ns’ means not significant at 95% confidence level.
Figure S1: A comparison of the viable cell density profile of HEK293T stable producer cell line culture in 24-DSW plates using same well sampling and sacrificial well sampling methods.