5 Conclusion

In summary, three hybridoma cell lines producing mAbs against ASFV p30 protein were obtained, and all of them we identified for the first time two novel epitopes (1MDFILNISMKMEVIFKTDLR20,26VFHAGSLYNW35) on p30 protein. Epitope mapping is an important technique used to determine regions within a protein that can be recognized by the immune system. Therefore, identifying the exact epitopes will create the opportunity to design epitope-based vaccines and genotype specific diagnostic tests.