COLLECTIONS AND SPAWNING OF ANIMALS
We collected mussel broodstock from high and low density sites within
each of three locations in Port Phillip Bay, Victoria, Australia during
the May winter spawning season. These included Williamstown
(-37.861695°, 144.912751°), Geelong (-38.148770°, 144.390864°) and
Portarlington (-38.111790°, 144.659556°). Mussels were transported to
the Victorian Marine Science Consortium research laboratories at
Queenscliff and held in flow-through tanks at ambient temperature (16
°C). All animals were cleaned of epiphytes and other debris and used for
spawning on the day of collection using the approach of Pettersen et al.
(2010).