2.10 RNA isolation and real-time PCR
Total RNA was extracted from fresh renal tissue by using the TRIzol method and then reverse transcribed into cDNA. The cDNA content was detected by real-time quantitative PCR to determine the gene transcription levels in the tissue. Samples from five independent experiments were utilized, and each sample was subjected to real-time PCR in triplicate. The primers used in this study are provided in Table S1.