2.5 Analysis of lipid peroxidation (LPO)
A Liperfluo assay kit was used to detect the lipid peroxide content in HK-2 cells. After washing three times, the cells were mixed with 1 μM Liperfluo, incubated in the dark at room temperature for 30 minutes, and then washed with PBS three times. Lipid peroxide was finally measured and evaluated by confocal microscopy.