2.4 Analysis of intracellular ROS levels
After planking and drug treatment for 24 h, the cells were mixed with DCFH-DA (1 μM) and incubated in the dark at room temperature for 30 minutes. After washing the cells with PBS three times, they were observed by a fluorescence microscope. The OD value was determined by an enzyme-labeling instrument, and the ROS content was determined.