2.7 Hematoxylin and eosin staining (HE)
Renal tissue portions were fixed overnight with 4% paraformaldehyde,
embedded in paraffin, sectioned, stained with HE, and then observed
under an optical microscope (Olympus, Japan). The degree of renal injury
was evaluated in three random fields per sample by grading the renal
tissue injury as follows: 0-10%, 0; 11-25%, 1; 26-50%, 2; 51-75%, 3;
and 76-100%, 4. At least five renal regions on each slide were randomly
selected.