DNA extraction and Sequencing
DNA was extracted from 68 inner-eggshell swabs using the NucleoSpin® 96 Soil extraction kit (Macherey-Nagel, Germany) following kit instructions. The PCR amplification of the TEF-1α gene was performed using Fusarium -specific primers with the cycling conditions reported previously (Cobo-Díaz et al., 2019). Eight inner-eggshell swabs (four-symptomatic and four-asymptomatic eggs) were sequenced following the Illumina MiSeq sequencing methodology as described by Menke et al. (2017). More detailed information is provided in supplementary material.