Figure 1 Pharmacological evidence of functional involvement of T-type channels in the development and maintenance of inflammation and related pain
The effect of acute TTA-A2 (1 mg/kg, per os ) or vehicle (VEH) treatment was assessed in mice 4h after an intraplantar injection of carrageenan (right ) or 7 days after an intra-articular administration of Complete Freund’s Adjuvant (CFA, middle ). In the CFA model, the effect of repeated (3 times a day from day 7 to day 14) administrations of TTA-A2 (1 mg/kg, per os ) or VEH was also investigated (left ). In all conditions, mechanical allodynia (A ), mechanical hyperalgesia (B ) and paw edema (C ) were assessed. Statistical differences were assessed by two-way ANOVA (Time, Treatments) followed by Bonferroni post-hoc test [significant differences between VEH and TTA-A2 group are indicated by asterisks (*p < 0.05; **p < 0.01; ***p < 0.001)]. Data represent mean ± SEM.