Figure 2 Genetic evidence for the functional involvement of Cav3.2 channels in the development of inflammation and related pain
Cav3.2 knock out mice (Cav3.2 KO) and their wild type littermates (WT) received an intraplantar administration of carrageenan (right ) or an intraarticular injection of CFA (left ). Mechanical allodynia (A ) and hyperalgesia (B ) were assessed by the von Frey test using 0.04 g and 1.4 g filaments, respectively. Edema size (C ) was measured with a caliper. IL-6 levels in edema (D ) was analyzed by ELISA in supernatant from culture of edema. Statistical differences were assessed by two-way ANOVA (Time, Genotype) followed by Bonferroni post-hoc test [significant differences between WT and Cav3.2 KO group are indicated by asterisks (*p < 0.05; **p< 0.01; ***p < 0.001)]. Statistical differences for IL-6 levels in edema were assessed by Mann-Whitney test [significant differences between WT and Cav3.2 KO group are indicated by asterisks (*p < 0.05; ***p < 0.001)]. Data represent mean ± SEM.