Bone-marrow derived macrophages and lymphocytes T
CD4+ cells
Bone-marrow derived macrophages (BMDM) and CD4+ T
cells were prepared from WT and
Cav3.2GFP-flox KI mice and used to
investigate the expression of Cav3.2 channels by
immunohistochemistry labelling. BMDM from WT and Cav3.2
KO mice was used to investigate the impact of Cav3.2
deletion on LPS-induced morphological modification, IL-6 secretion and
intracellular calcium by immunofluorescent labelling, ELISA, and calcium
imaging. CD4+ T cells from WT and
Cav3.2 KO mice were used to assess the proliferation
capacities after CD3/CD28 activation by flow cytometry.