2.3. LAMP assay using genomic DNA
LAMP reaction was performed in a 25- μl reaction mixture containing WarmStart Colorimetric LAMP 2X Master Mix, 2 μl DNA template, 8 μl nuclease free water, 1X primer mix. The LAMP primer mix consisted of 6 primers: two inner primers (1.6 μl, FIP and BIP), two outer primers (0.2 μl, F3 and B3), two loop primers (0.4 μl, LoopF and LoopB). The yaiO2 primer sequences were published in [22]. LAMP reactions were performed at 65 °C for 30 min. DNA integrity was checked using 1% agarose gel containing GelRed nucleic acid stain in 0.5X Tris-Borate-EDTA (TBE) buffer. Gel electrophoresis was run in the Mupid-One (Seraing, Belgium). Gel images were acquired using the BioRad™ GelDoc EZ Imaging Systems (California, USA).