Figure 1. LAMP device and Colony LAMP in the device
The thermally isolated mug maintains the temperature (Length: 11 cm,
radius: 7.5 cm, cost: 9 Euros). We designed a casing that houses the
printed circuit board and electronic components using SOLIDWORKS
software and manufactured it using a 3-dimensional (3D) printer and
Acrylonitrile Butadiene Styrene (ABS) filament (Length: 5 cm, width: 9.5
cm, depth: 6.5 cm). Total weight of the device was 70 g. We made an
array of PDMS microwells where LAMP reactions occurred (PDMS, Curing
reagent (1): Base (10)). We cured PDMS in a petri dish at 75◦C for 60 min. We punched 4x4 wells (6.25
cm2) using a 2.5-mm biopsy puncher (Robbins
Instruments, Chatham, MA, USA). The number of wells can be increased or
decreased according to required number of samples (Total area: 176.7
cm2). A PDMS slab was irreversibly bonded on a glass
slides using a Corona system (BD20-AC, Electro-Technic Products Inc.).
The components of the LAMP device with the steps of the experiment are
shown in Figure 1 . The principal block diagram for the circuit
of the system is illustrated in Figure 2.