2.1. Chemicals and reagents
Luria-Bertani (LB) medium was supplied by Sigma-Aldrich Shanghai Trading Co., Ltd. (Shanghai, China). 2x WarmStart Colorimetric LAMP kit and Phusion High-Fidelity PCR kit was provided by New England Biolabs (MA, USA). Bacterial strains of E. coli (ATCC10536) and P. aeruginosa (ATCC15442) were used. DNAzol® Reagent and Tris-HCl were purchased from ThermoFisher Scientific (Hayward, CA, USA). GelRed nucleic acid stain was ordered from Biotium (Hayward, CA, USA). Polydimethylsiloxane (PDMS) was ordered from Sylgard® 184 (Dow Corning, Midland, MI, USA). The yaiO2 primer set was purchased from Oligomer® (Ankara, TURKEY) [22].