3.3 Colony PCR
Finally, we performed a conventional Colony PCR using the F3 and B3 primers from the LAMP primer set. We selected medium-sized colonies ofE. coli (Rmin ≈ 1.5 mm, Rmean ≈ 1.8 mm, Rmax ≈ 2.5 mm) and P. aeruginosa (R ≈ 2 mm) strains. The Colony PCR reactions were performed in thermal cycler for 80 min (Experimental section, 2.5. Colony PCR ). We evaluated the results using electrophoresis, Figure 6 . The amplifiedyaiO gene sequence was 240 bp for E. coli samples, as expected, there was not any bands for the P. aeruginosa sample.