Figure Legends
Figure 1. LAMP device and Colony LAMP in the device
Figure 2 . Principal circuit diagram of the LAMP device.
Max6675: Thermocouple Interface, NODEMCU ESP32: Microcontroller, OLED
1306: Display, LM7805: Voltage Regulator
Figure 3 . Interface of the LAMP device through the server
Figure 4 . Colony-LAMP assay for E. coli and P.
aeruginosa colonies. a) Images of the colonies picked fromE. coli plate. R: Colony diameter, E. coli :
Rmin ≈ 1.437, Rmean ≈ 1.744,
Rmax ≈ 2.281 mm, P. aeruginosa :
Rmin ≈ 1.537, Rmean ≈ 1.853,
Rmax ≈ 2.012 mm. b) Colorimetric Colony-LAMP
reactions in the PDMS wells. Pink color indicates the negative, yellow
color shows the positive results. Electrophoresis results forc) E. coli, d) P. aeruginosa colonies.
L: DNA ladder, N: Negative control.
Figure 5 . Sensitivity and specificity of LAMP reactions using
genomic DNA. a) Colorimetric LAMP reactions in the PDMS wells.E. coli cells: 1% = 5.68x1010, 50% =
2.87x1011, 100% = 5.34x1011 copy
numbers. P. aeruginosa cells: 1% = 6.82x1010,
50% = 2.72x1011, 100% = 5.74x1011copy numbers. Pink color indicates the negative, yellow color shows the
positive results. b) Electrophoresis results for E. coliand P. aeruginosa strains. L: DNA ladder, N: Negative control.
Figure 6 Sensitivity and specificity of Colony PCR reactions.
L: DNA ladder, N: Negative sample, P: Positive sample, R: Colony
diameter. E. coli: Rmin ≈ 1.5 mm,
Rmean ≈ 1.8 mm, Rmax ≈ 2.5 mm. P.
aeruginosa : R ≈ 2 mm.