4. Conclusion
This study reported the first LAMP device that conducts Colony-LAMP
reactions at 65 ˚C for less than 30 min, allows carrying out 16 or more
reactions at the same time, operation parameters can be remotely
monitored, and results can be real-time visualized by naked eye while
the reactions are running. We performed LAMP, Colony-LAMP, and Colony
PCR reactions using E. coli and P. aeruginosa samples. We
presented detection of the yaiO gene which is specific toE. coli using the yaiO2 primer set. We presented
specificity and sensitivity of our approach using pure genomic DNA and
crude bacterial colonies. We benchmarked our Colony-LAMP detection
against Colony PCR. We strongly believe that our portable, remotely
controlled colorimetric-LAMP device with its low-cost, user-friendly
interface can greatly contribute rapid and reliable diagnosis in
versatile operational environments as clinics, fields, farms, and
resource-limited areas. Furthermore, our approach can be used for
on-field detection of pathogens without requiring a trained personnel or
expensive equipment. Considering the significant success of LAMP assays
in diagnosis sciences, we are inspired to integrate an automated nucleic
acid extraction module to this platform regardless of species of the
samples. To address this aim, we work on rapid and reliable platforms
for conducting nucleic acid extraction assays using mechanical and
chemical isolation methods those are safe not only for DNA amplification
and detection reactions but also to the user and environment.