Figure Legends
Figure 1. LAMP device and Colony LAMP in the device
Figure 2 . Principal circuit diagram of the LAMP device. Max6675: Thermocouple Interface, NODEMCU ESP32: Microcontroller, OLED 1306: Display, LM7805: Voltage Regulator
Figure 3 . Interface of the LAMP device through the server
Figure 4 . Colony-LAMP assay for E. coli and P. aeruginosa colonies. a) Images of the colonies picked fromE. coli plate. R: Colony diameter, E. coli : Rmin ≈ 1.437, Rmean ≈ 1.744, Rmax ≈ 2.281 mm, P. aeruginosa : Rmin ≈ 1.537, Rmean ≈ 1.853, Rmax ≈ 2.012 mm. b) Colorimetric Colony-LAMP reactions in the PDMS wells. Pink color indicates the negative, yellow color shows the positive results. Electrophoresis results forc) E. coli, d) P. aeruginosa colonies. L: DNA ladder, N: Negative control.
Figure 5 . Sensitivity and specificity of LAMP reactions using genomic DNA. a) Colorimetric LAMP reactions in the PDMS wells.E. coli cells: 1% = 5.68x1010, 50% = 2.87x1011, 100% = 5.34x1011 copy numbers. P. aeruginosa cells: 1% = 6.82x1010, 50% = 2.72x1011, 100% = 5.74x1011copy numbers. Pink color indicates the negative, yellow color shows the positive results. b) Electrophoresis results for E. coliand P. aeruginosa strains. L: DNA ladder, N: Negative control.
Figure 6 Sensitivity and specificity of Colony PCR reactions. L: DNA ladder, N: Negative sample, P: Positive sample, R: Colony diameter. E. coli: Rmin ≈ 1.5 mm, Rmean ≈ 1.8 mm, Rmax ≈ 2.5 mm. P. aeruginosa : R ≈ 2 mm.