Figure 1. LAMP device and Colony LAMP in the device
The thermally isolated mug maintains the temperature (Length: 11 cm, radius: 7.5 cm, cost: 9 Euros). We designed a casing that houses the printed circuit board and electronic components using SOLIDWORKS software and manufactured it using a 3-dimensional (3D) printer and Acrylonitrile Butadiene Styrene (ABS) filament (Length: 5 cm, width: 9.5 cm, depth: 6.5 cm). Total weight of the device was 70 g. We made an array of PDMS microwells where LAMP reactions occurred (PDMS, Curing reagent (1): Base (10)). We cured PDMS in a petri dish at 75C for 60 min. We punched 4x4 wells (6.25 cm2) using a 2.5-mm biopsy puncher (Robbins Instruments, Chatham, MA, USA). The number of wells can be increased or decreased according to required number of samples (Total area: 176.7 cm2). A PDMS slab was irreversibly bonded on a glass slides using a Corona system (BD20-AC, Electro-Technic Products Inc.). The components of the LAMP device with the steps of the experiment are shown in Figure 1 . The principal block diagram for the circuit of the system is illustrated in Figure 2.