Figure 3 . Interface of the LAMP device through the server
Since the LAMP reactions were sensitive to thermal changes, the controller parameters of the system were adjusted to provide precise temperature control. The steady-state temperature error of the system remained less than %1, Figure 3 .
3. Results and Discussion
Here, we conducted Colony-LAMP reactions directly using E. coliand P. aeruginosa bacterial colonies in our LAMP device. We used the yaiO2 primers to amplify the yaiO gene region, which is conserved across diverse lineages of E. coli , but not in anther foodborne pathogen such as P. aeruginosa. We evaluated the performance of the device and compared the obtained Colony-LAMP results to a conventional LAMP assay that is performed in the device using genomic DNA extracted from bacteria, and a Colony PCR in the thermal cycler. All results were determined through real-time monitoring of the color change of the reactions by naked eye and confirmed by electrophoresis.