2.4. Colony-LAMP assay
For the Colony-LAMP reaction, Warmstart Colorimetric LAMP 2X Master Mix,
primer mix, and nuclease free water were prepared in a 25-µl volume. A
colony was picked with a sterilized inoculating loop from the bacterial
plate and directly transferred into the LAMP reaction mixture. We
adjusted the pH of the reaction mixture to 8.8 with Tris-HCl in the
microwells of the PDMS slab. Then, the PDMS slab was transferred into
the LAMP device. The reactions were performed at 65 °C for 30 minutes.
Results of the reactions were determined by the color change under the
day light, while the positive reactions were in yellow
(~orange), the negative ones were in pink colors.