4. Conclusion
This study reported the first LAMP device that conducts Colony-LAMP reactions at 65 ˚C for less than 30 min, allows carrying out 16 or more reactions at the same time, operation parameters can be remotely monitored, and results can be real-time visualized by naked eye while the reactions are running. We performed LAMP, Colony-LAMP, and Colony PCR reactions using E. coli and P. aeruginosa samples. We presented detection of the yaiO gene which is specific toE. coli using the yaiO2 primer set. We presented specificity and sensitivity of our approach using pure genomic DNA and crude bacterial colonies. We benchmarked our Colony-LAMP detection against Colony PCR. We strongly believe that our portable, remotely controlled colorimetric-LAMP device with its low-cost, user-friendly interface can greatly contribute rapid and reliable diagnosis in versatile operational environments as clinics, fields, farms, and resource-limited areas. Furthermore, our approach can be used for on-field detection of pathogens without requiring a trained personnel or expensive equipment. Considering the significant success of LAMP assays in diagnosis sciences, we are inspired to integrate an automated nucleic acid extraction module to this platform regardless of species of the samples. To address this aim, we work on rapid and reliable platforms for conducting nucleic acid extraction assays using mechanical and chemical isolation methods those are safe not only for DNA amplification and detection reactions but also to the user and environment.