Figure 3 . Interface of the LAMP device through the server
Since the LAMP reactions were sensitive to thermal changes, the
controller parameters of the system were adjusted to provide precise
temperature control. The steady-state temperature error of the system
remained less than %1, Figure 3 .
3. Results and Discussion
Here, we conducted Colony-LAMP reactions directly using E. coliand P. aeruginosa bacterial colonies in our LAMP device. We used
the yaiO2 primers to amplify the yaiO gene
region, which is conserved across diverse lineages of E. coli ,
but not in anther foodborne pathogen such as P. aeruginosa. We
evaluated the performance of the device and compared the obtained
Colony-LAMP results to a conventional LAMP assay that is performed in
the device using genomic DNA extracted from bacteria, and a Colony PCR
in the thermal cycler. All results were determined through real-time
monitoring of the color change of the reactions by naked eye and
confirmed by electrophoresis.